Recombinant Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] KO Tested (ab182359)

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Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19085] to Phospholipase C beta 1/PLCB1
Suitable for: IHC-P, WB, IHC-Fr
Knockout validated
Reacts with: Mouse, Rat, Human

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Primary

Secondary

Protein

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Primary - Rabbit Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359)
IHC-P, WB, IHC-Fr

Secondary - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
ICC/IF, Flow Cyt, IHC-P, ELISA, IHC-Fr

Protein - Recombinant Human Phospholipase C beta 1/PLCB1 protein (ab153213)
ELISA, WB

View more associated products

Product name

Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085]
See all Phospholipase C beta 1/PLCB1 primary antibodies
Description

Rabbit monoclonal [EPR19085] to Phospholipase C beta 1/PLCB1
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WB, IHC-Frmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Mouse Phospholipase C beta 1/PLCB1 aa 1-300. The exact sequence is proprietary.
Database link: Q9Z1B3
Positive control
- WB: Human, mouse and rat brain lysates; HeLa, HepG2, NIH/3T3, C6 and PC-12 whole cell lysates. IHC-P: Human cerebrum, mouse hippocampus and rat cerebrum tissues. IHC-Fr: Mouse cerebrum tissue.
General notes
This product was previously labelled as Phospholipase C beta 1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19085
Isotype

IgG
Research areas

Alternative Versions
- Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human Plcb1 (Phospholipase C beta 1) knockout HeLa cell line (ab266033)
KO cell lysates
- Human Plcb1 (Phospholipase C beta 1) knockout HeLa cell lysate (ab257589)
Recombinant Protein
- Recombinant Human Phospholipase C beta 1/PLCB1 protein (ab153213)

Our Abpromise guarantee covers the use of ab182359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Detects a band of approximately 150, 100 kDa (predicted molecular weight: 138 kDa).
IHC-Fr		1/200. Perform antigen retrieval with heated citric acid solution (10mM citric acid pH 6.0 + 0.05% Tween-20).

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
Involvement in disease

Epileptic encephalopathy, early infantile, 12
Sequence similarities

Contains 1 C2 domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Cellular localization

Nucleus membrane. Cytoplasm. Colocalizes with the adrenergic receptors, ADREN1A and ADREN1B, at the nuclear membrane of cardiac myocytes.
Target information above from: UniProt accession Q9NQ66 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 23236 Human
- Entrez Gene: 18795 Mouse
- Entrez Gene: 24654 Rat
- Omim: 607120 Human
- SwissProt: Q9NQ66 Human
- SwissProt: Q9Z1B3 Mouse
- SwissProt: P10687 Rat
- Unigene: 431173 Human
- Unigene: 330607 Mouse
- Unigene: 45523 Rat
see all
Alternative names
- 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase beta 1 antibody
- 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate antibody
- 1-phosphatidylinositol 4 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase beta-1 antibody
- EIEE12 antibody
- Inositoltrisphosphohydrolase antibody
- Monophosphatidylinositol phosphodiesterase antibody
- Phosphb antibody
- Phosphoinositidase C antibody
- Phosphoinositide phospholipase C antibody
- Phosphoinositide phospholipase C-beta 1 antibody
- Phosphoinositide phospholipase C-beta-1 antibody
- Phospholipase C beta 1 (phosphoinositide-specific) antibody
- Phospholipase C I antibody
- Phospholipase C-beta-1 antibody
- Phospholipase C-I antibody
- PI PLC antibody
- PLC 1 antibody
- PLC beta 1 antibody
- PLC I antibody
- PLC-154 antibody
- PLC-beta-1 antibody
- PLC-I antibody
- PLC154 antibody
- Plcb antibody
- Plcb1 antibody
- Plcb1 protein antibody
- PLCB1_HUMAN antibody
- PLCbeta1 antibody
- Triphosphoinositide phosphodiesterase antibody
see all

Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359)

All lanes : Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PLCB1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Mouse heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 138 kDa
Observed band size: 150 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab182359 observed at 150 kDa. Red - loading control ab8245 observed at 36 kDa.

ab182359 Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] was shown to specifically react with Phospholipase C beta 1/PLCB1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266033 (knockout cell lysate ab257589) was used. Wild-type and Phospholipase C beta 1/PLCB1 knockout samples were subjected to SDS-PAGE. ab182359 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359)

All lanes : Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution

Lane 1 : Human brain lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 138 kDa
Observed band size: 100,150 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 8429045, 2468162).
Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359)

All lanes : Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 138 kDa
Observed band size: 100,150 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8429045, 2468162).
Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359)

All lanes : Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 138 kDa
Observed band size: 100,150 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 8429045, 2468162).
Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359)

All lanes : Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution

Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 138 kDa
Observed band size: 100,150 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 3minutes; Lane 2 and 3: 10 seconds.

The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 8429045, 2468162).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on normal Human cerebrum is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on normal Human colon. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359)

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human gastric cancer. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on mouse hippocampus is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on mouse kidney. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on rat cerebrum is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on rat kidney. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359)

Immunohistochemical analysis of paraffin-embedded frozen, 0.2% Triton X-100 permeabilized Mouse cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green).

The result showed cytoplasmic staining on mouse cerebrum.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

Publishing research using ab182359? Please let us know so that we can cite the reference in this datasheet.

ab182359 has been referenced in 1 publication.

Vivas O et al. Niemann-Pick Type C Disease Reveals a Link between Lysosomal Cholesterol and PtdIns(4,5)P2 That Regulates Neuronal Excitability. Cell Rep 27:2636-2648.e4 (2019). PubMed: 31141688

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Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

Recombinant Protein

Applications

Target

Function

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Certificate of Compliance

References (1)

Customer reviews and Q&As