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Rabbit monoclonal [EPR1498Y] to Phospholipase C beta 3 (phospho S537)
This antibody detects Phospholipase C beta 3 phosphorylated on Ser537.
WBmore detailsUnsuitable for:
Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP
Phosphor-specific peptide corresponding to residues surrounding Serine 537 of human Phospholipase C beta 3.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP.
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
Contains 1 C2 domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Information by UniProt
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase beta-3 antibody
Western blot - Anti-Phospholipase C beta 3 (phospho S537) antibody [EPR1498Y] (ab51247)
All lanes :
Anti-Phospholipase C beta 3 (phospho S537) antibody [EPR1498Y] (ab51247) at 1/2000 dilutionLane 1 :
HeLa cell lysate, untreatedLane 2 :
HeLa cell lysate, treated with TPA
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat anti-rabbit HRP labeled at 1/2000 dilutionPredicted band size:
139 kDaObserved band size:
150 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"