Product nameAnti-Phospholipase C beta 3/PLCB3 antibody [EPR5951]
See all Phospholipase C beta 3/PLCB3 primary antibodies
DescriptionRabbit monoclonal [EPR5951] to Phospholipase C beta 3/PLCB3
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
corresponding to Human Phospholipase C beta 3/PLCB3 aa 1150-1250.
- HCT 116, 293T, and A431 cell lysates; Human breast and colon tissues.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Phospholipase C beta 3
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.|
FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
- Information by UniProt
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase beta-3 antibody
All lanes : Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (ab124735) at 1/1000 dilution
Lane 1 : HCT 116 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 139 kDa
ab124735 at 1/100 dilution staining Phospholipase C beta 3/PLCB3 in paraffin-embedded Human breast tissue by Immunohistochemistry.
ab124735 at 1/100 dilution staining Phospholipase C beta 3/PLCB3 in paraffin-embedded Human colon tissue by Immunohistochemistry.
ab124735 has not yet been referenced specifically in any publications.