Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1498Y] to Phospholipase C beta 3/PLCB3 (phospho S537)
- Suitable for: WB
- Reacts with: Human
Product nameAnti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y]
See all Phospholipase C beta 3/PLCB3 primary antibodies
DescriptionRabbit monoclonal [EPR1498Y] to Phospholipase C beta 3/PLCB3 (phospho S537)
This antibody detects Phospholipase C beta 3/PLCB3 phosphorylated on Ser537.
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP
Species reactivityReacts with: Human
corresponding to Human Phospholipase C beta 3/PLCB3 aa 500-600.
- HeLa whole cell lysate (ab150035).
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Phospholipase C beta 3.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab51247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).|
FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
- Information by UniProt
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase beta-3 antibody
All lanes : Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y] (ab51247) at 1/2000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with TPA
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 139 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab51247 has not yet been referenced specifically in any publications.