Product nameAnti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y]
See all Phospholipase C beta 3/PLCB3 primary antibodies
DescriptionRabbit monoclonal [EPR1498Y] to Phospholipase C beta 3/PLCB3 (phospho S537)
This antibody detects Phospholipase C beta 3/PLCB3 phosphorylated on Ser537.
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP
Species reactivityReacts with: Human
corresponding to Human Phospholipase C beta 3/PLCB3 aa 500-600.
- HeLa whole cell lysate (ab150035).
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Phospholipase C beta 3
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab51247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).|
FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
- Information by UniProt
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase beta-3 antibody
All lanes : Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y] (ab51247) at 1/2000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with TPA
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 139 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
ab51247 has not yet been referenced specifically in any publications.