The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 148 kDa.
It should be user determined
PLC (Phosphoinositide-specific phospholipase C) plays a significant role in transmembrane signaling. Four members of PLCs have been identified: PLCß, PLCg, PLCd, and PLCe. In response to extracellular stimuli (e.g., hormone, growth factors, neurotransmitters), PLC hydrolizes phosphatidylinositol 4,5-biphosphate (PIP2) into two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PLCg2 is engaged in antigen dependent signaling in B-cells and collagen-dependent signaling in platelets.