Key features and details
- Rabbit polyclonal to Phospholipase C gamma 1 + 2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Phospholipase C gamma 1 + 2 antibody
DescriptionRabbit polyclonal to Phospholipase C gamma 1 + 2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Phospholipase C gamma 1 + 2. It surrounds amino acid 1252 of Phospholipase C gamma 2.
Database link: P16885
- Jurkat whole cell lysate (ab7899), Human brain cortex tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.5% BSA, 69% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab174787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 148 kDa.
It should be user determined
RelevancePLC (Phosphoinositide-specific phospholipase C) plays a significant role in transmembrane signaling. Four members of PLCs have been identified: PLCß, PLCg, PLCd, and PLCe. In response to extracellular stimuli (e.g., hormone, growth factors, neurotransmitters), PLC hydrolizes phosphatidylinositol 4,5-biphosphate (PIP2) into two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PLCg2 is engaged in antigen dependent signaling in B-cells and collagen-dependent signaling in platelets.
Cellular localizationCytoplasmic and Plasma membrane
- 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase gamma 1 antibody
- 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate antibody
ab174787 has not yet been referenced specifically in any publications.