Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) antibody (ab41434)
Key features and details
- Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) antibody
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies -
Description
Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) -
Host species
Rabbit -
Specificity
Phospho-Tyr775-\Phospholipase C gamma 1/PLC-gamma-1 specific. Immunogen sequence has low homology to Phospholipase C gamma 2.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775).
Database link: P19174 -
Positive control
- Human Jurkat and A431 cells treated with pervanadate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was cross-adsorbed to a non-specific phospho-tyrosine peptide, then affinity purified using phospho- Phospholipase C gamma 1/PLC-gamma-1 (Tyr775) peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa). |
Target
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Function
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Domain
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
Post-translational
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
Cellular localization
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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Database links
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- SwissProt: P10686 Rat
- Unigene: 268177 Human
see all -
Alternative names
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
Images
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Western blot analysis of Phospholipase C gamma 1/PLC-gamma-1 immunoprecipitates from human jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2, 4, 5 & 6). Immunoprecipitation was performed with monoclonal anti-Phospholipase C gamma 1/PLC-gamma-1. The blots were probed with anti- Phospholipase C gamma 1/PLC-gamma-1 (lanes 1 & 2) and anti-Phospholipase C gamma 1/PLC-gamma-1 (Tyr-775) (lanes 3-6). The latter antibody was used in the presence of phospho- Phospholipase C gamma 1/PLC-gamma-1 (Tyr-775) peptide (lane 5), or a non-specific phosphotyrosine peptide (lane 6).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab41434 has not yet been referenced specifically in any publications.