Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody (ab107455)
Key features and details
- Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies -
Description
Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1 aa 700-800 conjugated to keyhole limpet haemocyanin.
(Peptide available asab129952) -
Positive control
- This antibody gave a positive signal in both Human Brain and Spinal Cord tissue lysates as well as the following whole cell lysates: HeLa; HEK293; HepG2; Jurkat; MCF7. It also gave a positive signal in HepG2 and MCF7 cell lines in IF.
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General notes
Previously labelled as Phospholipase C gamma 1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab107455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa). |
Target
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Function
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Domain
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
Post-translational
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
Cellular localization
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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Database links
- Entrez Gene: 281987 Cow
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P08487 Cow
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
see all -
Alternative names
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
Images
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody (ab107455)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Phospholipase C gamma 1 / PLC-gamma-1 knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: Mouse brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab107455 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab107455 was shown to recognize Phospholipase C gamma 1 / PLC-gamma-1 when Phospholipase C gamma 1 / PLC-gamma-1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1 / PLC-gamma-1 knockout samples were subjected to SDS-PAGE. ab107455 at a concentration of 1 µg/ml and ab8245 (loading control to GAPDH) at a dilution of 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody (ab107455)All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody (ab107455) at 1 µg/ml (Milk Blocking - 3%)
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 148 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa, 57 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody (ab107455)ICC/IF image of ab107455 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab107455, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 cells at 5µg/ml.
Protocols
Datasheets and documents
References (0)
ab107455 has not yet been referenced specifically in any publications.
