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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)

  • Datasheet
  • SDS
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
  • Flow Cytometry - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
  • Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5358] to Phospholipase C gamma 1/PLC-gamma-1
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

You may also be interested in

Protein
Product image
Recombinant Human Phospholipase C gamma 1/PLC-gamma-1 protein (ab112339)
Knockout
Product image
Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate (ab257590)
Knockout
Product image
Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell line (ab266530)

View more associated products

Overview

  • Product name

    Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358]
    See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5358] to Phospholipase C gamma 1/PLC-gamma-1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293-T, HepG2, Jurkat, and MCF-7 cell lysates. Flow Cyt: Jurkat cells.
  • General notes

    This product was previously labelled as Phospholipase C gamma 1. 

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5358
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Hydrolysis
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLC
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Hydrolysis
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] - BSA and Azide free (ab247882)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human Phospholipase C gamma 1/PLC-gamma-1 protein (ab112339)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Mouse
Human
All applications
Rat
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 149 kDa.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 149 kDa.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
    • Sequence similarities

      Contains 1 C2 domain.
      Contains 1 EF-hand domain.
      Contains 2 PH domains.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • Domain

      The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
    • Post-translational
      modifications

      The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
      Ubiquitinated by CBLB in activated T-cells.
    • Cellular localization

      Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
    • Target information above from: UniProt accession P19174 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5335 Human
      • Entrez Gene: 18803 Mouse
      • Entrez Gene: 25738 Rat
      • Omim: 172420 Human
      • SwissProt: P19174 Human
      • SwissProt: Q62077 Mouse
      • SwissProt: P10686 Rat
      • Unigene: 268177 Human
      • Unigene: 44463 Mouse
      • Unigene: 11243 Rat
      see all
    • Alternative names

      • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
      • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
      • Inositoltrisphosphohydrolase antibody
      • Monophosphatidylinositol phosphodiesterase antibody
      • NCKAP3 antibody
      • Phosphatidylinositol phospholipase C antibody
      • Phosphoinositidase C antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-1 antibody
      • Phospholipase C 148 antibody
      • Phospholipase C gamma 1 antibody
      • Phospholipase C-gamma-1 antibody
      • Phospholipase C-II antibody
      • PLC gamma 1 antibody
      • PLC II antibody
      • PLC-148 antibody
      • PLC-gamma-1 antibody
      • PLC-II antibody
      • PLC1 antibody
      • PLC148 antibody
      • Plcg1 antibody
      • PLCG1_HUMAN antibody
      • PLCgamma1 antibody
      see all

    Images

    • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
      Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
      All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293T cell lysate
      Lane 2 : PLCG1 knockout HEK-293T cell lysate

      Lysates/proteins at 40 µg/ml per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution

      Predicted band size: 149 kDa



      Lanes 1- 2: Merged signal (red and green). Green - ab109501 observed at 160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

      ab109501 was shown to react with Phospholipase C gamma 1/PLC-gamma-1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266530 (knockout cell lysate ab257590) was used. Wild-type HEK-293T and PLCG1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109501 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
      Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Phospholipase C gamma 1 / PLC-gamma-1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HepG2 cell lysate (20 µg)
      Lane 4: Mouse brain tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109501 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109501 was shown to recognize Phospholipase C gamma 1 / PLC-gamma-1 when Phospholipase C gamma 1 / PLC-gamma-1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1 / PLC-gamma-1 knockout samples were subjected to SDS-PAGE. ab109501 at a dilution of 1/1000 and ab8245 (loading control to GAPDH) at a dilution of 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
      Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
      All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : MCF-7 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 149 kDa

    • Flow Cytometry - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
      Flow Cytometry - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
      Flow cytometric analysis of permeabilized Jurkat cells using ab109501 at 1/10 dilution (red)or a rabbit IgG (negative) (green).
    • Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
      Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab109501? Please let us know so that we can cite the reference in this datasheet.

    ab109501 has not yet been referenced specifically in any publications.

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