Recombinant Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
![Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)](https://www.abcam.com/ps/products/109/ab109501/Images/ab109501-395787-anti-phospholipase-c-gamma-1plc-gamma-1-antibody-epr5358-western-blot-hek-293t-lysates.jpg "Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5358] to Phospholipase C gamma 1/PLC-gamma-1
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358]
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies -
Description
Rabbit monoclonal [EPR5358] to Phospholipase C gamma 1/PLC-gamma-1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293-T, HepG2, Jurkat, and MCF-7 cell lysates. Flow Cyt (intra): Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5358 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 149 kDa.
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| Notes |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 149 kDa. |
Target
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Function
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Domain
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
Post-translational
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
Cellular localization
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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Database links
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- SwissProt: P10686 Rat
- Unigene: 268177 Human
see all -
Alternative names
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
Images
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PLCG1 knockout HEK-293T cell lysate
Lysates/proteins at 40 µg/ml per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Predicted band size: 149 kDaLanes 1- 2: Merged signal (red and green). Green - ab109501 observed at 160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab109501 was shown to react with Phospholipase C gamma 1/PLC-gamma-1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266530 (knockout cell lysate ab257590) was used. Wild-type HEK-293T and PLCG1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109501 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)](https://www.abcam.com/ps/products/109/ab109501/Images/ab109501-259086-ab109501KOWB.jpg)
Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Phospholipase C gamma 1 / PLC-gamma-1 knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: Mouse brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109501 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109501 was shown to recognize Phospholipase C gamma 1 / PLC-gamma-1 when Phospholipase C gamma 1 / PLC-gamma-1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1 / PLC-gamma-1 knockout samples were subjected to SDS-PAGE. ab109501 at a dilution of 1/1000 and ab8245 (loading control to GAPDH) at a dilution of 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)](https://www.abcam.com/ps/products/109/ab109501/Images/Phospholipase-C-gamma-1-Primary-antibodies-ab109501-2.jpg)
Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF-7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 149 kDa -
![Flow Cytometry (Intracellular) - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)](https://www.abcam.com/ps/products/109/ab109501/Images/Phospholipase-C-gamma-1-Primary-antibodies-ab109501-4.jpg)
Flow Cytometry (Intracellular) - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)Intracellular flow cytometric analysis of permeabilized Jurkat cells using ab109501 at 1/10 dilution (red)or a rabbit IgG (negative) (green).
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![Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)](https://www.abcam.com/ps/products/109/ab109501/Images/ab109501-5-benefits-of-recombinant-antibodies.png)
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
Datasheets and documents
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Datasheet download
References (0)
ab109501 has not yet been referenced specifically in any publications.
