Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho S1248) antibody - C-terminal (ab226966)

Overview

  • Product name

    Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho S1248) antibody - C-terminal
    See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies
  • Description

    Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho S1248) - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Phospholipase C gamma 1/PLC-gamma-1 (C terminal) (phospho S1248). The exact sequence is proprietary.
    Database link: P19174

  • Positive control

    • WB: Starved A431, whole cell extracts. Starved A431 treated with EGF, whole cell extracts.
  • General notes

    Previously labelled as Phospholipase C gamma 1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab226966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000.

Target

  • Function

    Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 EF-hand domain.
    Contains 2 PH domains.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Domain

    The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
  • Post-translational
    modifications

    The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
    Ubiquitinated by CBLB in activated T-cells.
  • Cellular localization

    Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
    • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
    • Inositoltrisphosphohydrolase antibody
    • Monophosphatidylinositol phosphodiesterase antibody
    • NCKAP3 antibody
    • Phosphatidylinositol phospholipase C antibody
    • Phosphoinositidase C antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-gamma-1 antibody
    • Phospholipase C 148 antibody
    • Phospholipase C gamma 1 antibody
    • Phospholipase C-gamma-1 antibody
    • Phospholipase C-II antibody
    • PLC gamma 1 antibody
    • PLC II antibody
    • PLC-148 antibody
    • PLC-gamma-1 antibody
    • PLC-II antibody
    • PLC1 antibody
    • PLC148 antibody
    • Plcg1 antibody
    • PLCG1_HUMAN antibody
    • PLCgamma1 antibody
    see all

Images

  • All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho S1248) antibody - C-terminal (ab226966) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) cells starved for 16 hours, whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) cells starved for 16 hours then treated with 100 ng/ml EGF for 5 minutes, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Developed using the ECL technique.


    7.5% SDS-PAGE

References

ab226966 has not yet been referenced specifically in any publications.

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