Product nameAnti-Phospholipase C gamma 1/PLC-gamma-1 (phospho S1248) antibody - C-terminal
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies
DescriptionRabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho S1248) - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Phospholipase C gamma 1/PLC-gamma-1 (C terminal) (phospho S1248). The exact sequence is proprietary.
Database link: P19174
- WB: Starved A431, whole cell extracts. Starved A431 treated with EGF, whole cell extracts.
Previously labelled as Phospholipase C gamma 1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab226966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000.|
FunctionPlays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
Sequence similaritiesContains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
DomainThe SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells.
Cellular localizationCell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
- Information by UniProt
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho S1248) antibody - C-terminal (ab226966) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) cells starved for 16 hours, whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) cells starved for 16 hours then treated with 100 ng/ml EGF for 5 minutes, whole cell extract
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
ab226966 has not yet been referenced specifically in any publications.