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Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455)
  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455)

Key features and details

  • Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771)
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Protein
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Recombinant Human Phospholipase C gamma 1/PLC-gamma-1 protein (ab112339)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
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Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell line (ab266530)

View more associated products

Overview

  • Product name

    Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody
    See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies
  • Description

    Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1.
    Sequence:

    P-D-Y(p)-G-A


    Database link: NP_002651.2
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Methanol fixed HeLa cells; 3T3 cell extracts.
  • General notes

    Previously labelled as Phospholipase C gamma 1. 

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibodies were purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Hydrolysis
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLC
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Hydrolysis

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human Phospholipase C gamma 1/PLC-gamma-1 protein (ab112339)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Mouse
Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 148 kDa.
ICC/IF
1/100 - 1/200.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 148 kDa.
ICC/IF
1/100 - 1/200.

Target

  • Function

    Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 EF-hand domain.
    Contains 2 PH domains.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Domain

    The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
  • Post-translational
    modifications

    The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
    Ubiquitinated by CBLB in activated T-cells.
  • Cellular localization

    Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
  • Target information above from: UniProt accession P19174 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5335 Human
    • Entrez Gene: 18803 Mouse
    • Omim: 172420 Human
    • SwissProt: P19174 Human
    • SwissProt: Q62077 Mouse
    • Unigene: 268177 Human
    • Unigene: 44463 Mouse
    • Alternative names

      • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
      • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
      • Inositoltrisphosphohydrolase antibody
      • Monophosphatidylinositol phosphodiesterase antibody
      • NCKAP3 antibody
      • Phosphatidylinositol phospholipase C antibody
      • Phosphoinositidase C antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-1 antibody
      • Phospholipase C 148 antibody
      • Phospholipase C gamma 1 antibody
      • Phospholipase C-gamma-1 antibody
      • Phospholipase C-II antibody
      • PLC gamma 1 antibody
      • PLC II antibody
      • PLC-148 antibody
      • PLC-gamma-1 antibody
      • PLC-II antibody
      • PLC1 antibody
      • PLC148 antibody
      • Plcg1 antibody
      • PLCG1_HUMAN antibody
      • PLCgamma1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455)
      Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455)

      Immunofluorescence staining of methanol fixed HeLa cells labelling Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) with ab131455 antibody at a dilution of 1/100.

    • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455)
      Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455)
      All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455) at 1/500 dilution

      Lane 1 : 3T3 cell extracts untreated
      Lane 2 : 3T3 cell extracts serum treated

      Predicted band size: 148 kDa

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab131455? Please let us know so that we can cite the reference in this datasheet.

    ab131455 has been referenced in 1 publication.

    • Kang DS  et al. Netrin-1/DCC-mediated PLC?1 activation is required for axon guidance and brain structure development. EMBO Rep 19:N/A (2018). PubMed: 30224412

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