Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455)
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Overview
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Product name
Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies -
Description
Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) -
Host species
Rabbit -
Specificity
ab131455 detects endogenous levels of Phospholipase C gamma 1/PLC-gamma-1 only when phosphorylated at Tyrosine 771.
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Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1.
Sequence:P-D-Y(p)-G-A
Database link: NP_002651.2 -
Positive control
- Methanol fixed HeLa cells; 3T3 cell extracts.
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General notes
Previously labelled as Phospholipase C gamma 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab131455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 148 kDa. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Domain
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
Post-translational
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
Cellular localization
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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Database links
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- SwissProt: P10686 Rat
- Unigene: 268177 Human
see all -
Alternative names
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455)Immunofluorescence staining of methanol fixed HeLa cells labelling Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) with ab131455 antibody at a dilution of 1/100.
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455)All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455) at 1/500 dilution
Lane 1 : 3T3 cell extracts untreated
Lane 2 : 3T3 cell extracts serum treated
Predicted band size: 148 kDa
Datasheets and documents
References
ab131455 has not yet been referenced specifically in any publications.
