Product nameAnti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies
DescriptionRabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771)
ab131455 detects endogenous levels of Phospholipase C gamma 1/PLC-gamma-1 only when phosphorylated at Tyrosine 771.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- Methanol fixed HeLa cells; 3T3 cell extracts.
Previously labelled as Phospholipase C gamma 1.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab131455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 148 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionPlays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
Sequence similaritiesContains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
DomainThe SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells.
Cellular localizationCell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
- Information by UniProt
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
Immunofluorescence staining of methanol fixed HeLa cells labelling Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) with ab131455 antibody at a dilution of 1/100.
All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455) at 1/500 dilution
Lane 1 : 3T3 cell extracts untreated
Lane 2 : 3T3 cell extracts serum treated
Predicted band size: 148 kDa
ab131455 has not yet been referenced specifically in any publications.