Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455)
Key features and details
- Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies -
Description
Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1.
Sequence:P-D-Y(p)-G-A
Database link: NP_002651.2 -
Positive control
- Methanol fixed HeLa cells; 3T3 cell extracts.
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General notes
Previously labelled as Phospholipase C gamma 1.
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Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 148 kDa.
|
|
| ICC/IF |
1/100 - 1/200.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 148 kDa. |
|
ICC/IF
1/100 - 1/200. |
Target
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Function
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Domain
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
Post-translational
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
Cellular localization
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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Database links
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- Unigene: 268177 Human
- Unigene: 44463 Mouse
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Alternative names
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455)
Immunofluorescence staining of methanol fixed HeLa cells labelling Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) with ab131455 antibody at a dilution of 1/100.
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455)All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y771) antibody (ab131455) at 1/500 dilution
Lane 1 : 3T3 cell extracts untreated
Lane 2 : 3T3 cell extracts serum treated
Predicted band size: 148 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab131455 has been referenced in 1 publication.
- Kang DS et al. Netrin-1/DCC-mediated PLC?1 activation is required for axon guidance and brain structure development. EMBO Rep 19:N/A (2018). PubMed: 30224412