Product nameAnti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] - BSA free
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies
DescriptionRabbit monoclonal [EP1898Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) - BSA free
Tested applicationsSuitable for: WB, ICC/IF, Dot blotmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1.
- WB: Jurkat cell lysates treated with pervanadate. ICC/IF: Jurkat cells treated with pervanadate.
The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab264087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).|
|ICC/IF||Use at an assay dependent dilution.|
|Dot blot||Use at an assay dependent dilution.|
FunctionPlays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
Sequence similaritiesContains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
DomainThe SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells.
Cellular localizationCell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
- Information by UniProt
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264087 has not yet been referenced specifically in any publications.