Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2611Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
- Suitable for: WB
- Reacts with: Human
Product nameAnti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y]
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies
DescriptionRabbit monoclonal [EPR2611Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
This antibody detects Phospholipase C gamma 1/PLC-gamma-1 phosphorylated on Tyrosine 783.
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783).
Database link: P19174
- WB: Jurkat cell lysate treated with Pervanadate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Phospholipase C gamma 1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab134186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).|
FunctionPlays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
Sequence similaritiesContains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
DomainThe SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells.
Cellular localizationCell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
- Information by UniProt
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] (ab134186) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate
treated with Pervanadate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 148 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
ab134186 has been referenced in 1 publication.
- Xie L et al. FGFR2 gene amplification in gastric cancer predicts sensitivity to the selective FGFR inhibitor AZD4547. Clin Cancer Res 19:2572-83 (2013). PubMed: 23493349