Recombinant
RabMAb

Recombinant Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] - BSA and Azide free (ab248714)

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Overview

  • Product name

    Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] - BSA and Azide free
    See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2611Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783). The exact sequence is proprietary.
    Database link: P19174

  • General notes

    ab248714 is the carrier-free version of ab134186 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248714 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab248714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function

      Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.

    • Sequence similarities

      Contains 1 C2 domain.
      Contains 1 EF-hand domain.
      Contains 2 PH domains.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.

    • Domain

      The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.

    • Post-translational
      modifications

      The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
      Ubiquitinated by CBLB in activated T-cells.

    • Cellular localization

      Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
    • Information by UniProt

    • Database links

      • Alternative names

        • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
        • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
        • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
        • Inositoltrisphosphohydrolase antibody
        • Monophosphatidylinositol phosphodiesterase antibody
        • NCKAP3 antibody
        • Phosphatidylinositol phospholipase C antibody
        • Phosphoinositidase C antibody
        • Phosphoinositide phospholipase C antibody
        • Phosphoinositide phospholipase C-gamma-1 antibody
        • Phospholipase C 148 antibody
        • Phospholipase C gamma 1 antibody
        • Phospholipase C-gamma-1 antibody
        • Phospholipase C-II antibody
        • PLC gamma 1 antibody
        • PLC II antibody
        • PLC-148 antibody
        • PLC-gamma-1 antibody
        • PLC-II antibody
        • PLC1 antibody
        • PLC148 antibody
        • Plcg1 antibody
        • PLCG1_HUMAN antibody
        • PLCgamma1 antibody
        see all

      References

      ab248714 has not yet been referenced specifically in any publications.

      Publishing research using ab248714? Please let us know so that we can cite the reference in this datasheet.

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