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RecombinantRabMAb

Recombinant Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] - BSA and Azide free (ab248714)

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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] - BSA and Azide free (ab248714)
  • Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] - BSA and Azide free (ab248714)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2611Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

You may also be interested in

Conjugation
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HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
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Recombinant Human Phospholipase C gamma 1/PLC-gamma-1 protein (ab112339)

View more associated products

Overview

  • Product name

    Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] - BSA and Azide free
    See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2611Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783). The exact sequence is proprietary.
    Database link: P19174

  • General notes

    ab248714 is the carrier-free version of ab134186 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248714 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2611Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Hydrolysis
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLC
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Hydrolysis

Associated products

  • Alternative Versions

    • Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] (ab134186)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant Human Phospholipase C gamma 1/PLC-gamma-1 protein (ab112339)

Applications

Our Abpromise guarantee covers the use of ab248714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
    • Sequence similarities

      Contains 1 C2 domain.
      Contains 1 EF-hand domain.
      Contains 2 PH domains.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • Domain

      The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
    • Post-translational
      modifications

      The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
      Ubiquitinated by CBLB in activated T-cells.
    • Cellular localization

      Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
    • Target information above from: UniProt accession P19174 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5335 Human
      • Omim: 172420 Human
      • SwissProt: P19174 Human
      • Unigene: 268177 Human
      • Alternative names

        • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
        • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
        • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
        • Inositoltrisphosphohydrolase antibody
        • Monophosphatidylinositol phosphodiesterase antibody
        • NCKAP3 antibody
        • Phosphatidylinositol phospholipase C antibody
        • Phosphoinositidase C antibody
        • Phosphoinositide phospholipase C antibody
        • Phosphoinositide phospholipase C-gamma-1 antibody
        • Phospholipase C 148 antibody
        • Phospholipase C gamma 1 antibody
        • Phospholipase C-gamma-1 antibody
        • Phospholipase C-II antibody
        • PLC gamma 1 antibody
        • PLC II antibody
        • PLC-148 antibody
        • PLC-gamma-1 antibody
        • PLC-II antibody
        • PLC1 antibody
        • PLC148 antibody
        • Plcg1 antibody
        • PLCG1_HUMAN antibody
        • PLCgamma1 antibody
        see all

      Images

      • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] - BSA and Azide free (ab248714)
        Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] - BSA and Azide free (ab248714)
        All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] (ab134186) at 1/10000 dilution

        Lane 1 : Jurkat cell lysate untreated
        Lane 2 : Jurkat cell lysate treated with Pervanadate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 148 kDa
        Observed band size: 150 kDa
        why is the actual band size different from the predicted?



        This data was developed using ab134186, the same antibody clone in a different buffer formulation.

         

      • Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] - BSA and Azide free (ab248714)
        Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] - BSA and Azide free (ab248714)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab248714? Please let us know so that we can cite the reference in this datasheet.

      ab248714 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab248714.
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