Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [PLCG1Y783-C4] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
Related conjugates and formulations
Product nameAnti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4]
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies
DescriptionRabbit monoclonal [PLCG1Y783-C4] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P19174
- Flow cyt: HeLa cells (treated with pervanadate); L929 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
PurityProtein A/G purified
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab278707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.001 - 1 µg/ml.
Use a concentration of 0.001 - 1 µg/ml.
FunctionPlays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
Sequence similaritiesContains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
DomainThe SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells.
Cellular localizationCell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
- Information by UniProt
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.01 µg/mL ab278707.
ab278707 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of ab278707.
Peptide blockage flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) treated with imatinib (red) treated with pervanadate (green) treated with PV + blocked with non-phospho- peptide (violet) or treated with PV + blocked with phospho-peptide (brown) using ab278707 at 0.05 µg/mL.
Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using ab278707 at 0.1 ug/mL.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab278707 has not yet been referenced specifically in any publications.