Anti-Phospholipase D1 antibody (ab240563)
Key features and details
- Rabbit polyclonal to Phospholipase D1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Phospholipase D1 antibody
See all Phospholipase D1 primary antibodies -
Description
Rabbit polyclonal to Phospholipase D1 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PLD1 aa 525-575. The exact sequence is proprietary.
Database link: Q13393 -
Positive control
- IP: HeLa whole cell lysate.
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General notes
Previously labelled as Phospholipase D.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab240563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic. -
Tissue specificity
Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine. -
Sequence similarities
Belongs to the phospholipase D family.
Contains 1 PH domain.
Contains 2 PLD phosphodiesterase domains.
Contains 1 PX (phox homology) domain. -
Cellular localization
Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 5337 Human
- Omim: 602382 Human
- SwissProt: Q13393 Human
- Unigene: 382865 Human
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Alternative names
- Choline phosphatase 1 antibody
- hPLD1 antibody
- Phosphatidylcholine-hydrolyzing phospholipase D1 antibody
see all
Images
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Immunoprecipitation - Anti-Phospholipase D1 antibody (ab240563)
PLD1 was immunoprecipitated from 0.5 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) using ab240563 at 6 μg/mg lysate. Western blot was perfromed on the immunoprecipitate using ab240563 at 0.4 μg/ml.
Lane 1: ab240563 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab240563 has not yet been referenced specifically in any publications.
