Anti-Phospholipase D1 antibody (ab240563)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Phospholipase D1 antibody
See all Phospholipase D1 primary antibodies -
Description
Rabbit polyclonal to Phospholipase D1 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PLD1 aa 525-575. The exact sequence is proprietary.
Database link: Q13393 -
Positive control
- IP: HeLa whole cell lysate.
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General notes
Previously labelled as Phospholipase D.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab240563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic. -
Tissue specificity
Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine. -
Sequence similarities
Belongs to the phospholipase D family.
Contains 1 PH domain.
Contains 2 PLD phosphodiesterase domains.
Contains 1 PX (phox homology) domain. -
Cellular localization
Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 5337 Human
- Omim: 602382 Human
- SwissProt: Q13393 Human
- Unigene: 382865 Human
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Alternative names
- Choline phosphatase 1 antibody
- hPLD1 antibody
- Phosphatidylcholine-hydrolyzing phospholipase D1 antibody
see all
Images
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Immunoprecipitation - Anti-Phospholipase D1 antibody (ab240563)PLD1 was immunoprecipitated from 0.5 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) using ab240563 at 6 μg/mg lysate. Western blot was perfromed on the immunoprecipitate using ab240563 at 0.4 μg/ml.
Lane 1: ab240563 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 3 minutes.
Datasheets and documents
References
ab240563 has not yet been referenced specifically in any publications.
