Key features and details
- Rabbit polyclonal to Phospholipase D1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Phospholipase D1 antibody
See all Phospholipase D1 primary antibodies
DescriptionRabbit polyclonal to Phospholipase D1
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PLD1 aa 525-575. The exact sequence is proprietary.
Database link: Q13393
- IP: HeLa whole cell lysate.
Previously labelled as Phospholipase D.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
Our Abpromise guarantee covers the use of ab240563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionImplicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.
Tissue specificityExpressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
Sequence similaritiesBelongs to the phospholipase D family.
Contains 1 PH domain.
Contains 2 PLD phosphodiesterase domains.
Contains 1 PX (phox homology) domain.
Cellular localizationCytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane.
- Information by UniProt
- Choline phosphatase 1 antibody
- hPLD1 antibody
- Phosphatidylcholine-hydrolyzing phospholipase D1 antibody
PLD1 was immunoprecipitated from 0.5 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) using ab240563 at 6 μg/mg lysate. Western blot was perfromed on the immunoprecipitate using ab240563 at 0.4 μg/ml.
Lane 1: ab240563 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240563 has not yet been referenced specifically in any publications.