Anti-Phospholipase D1 antibody [EP1506Y] (ab68150)
![Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (ab68150)](https://www.abcam.com/ps/products/68/ab68150/Images/ab68150-82360-anti-phospholipase-d1-antibody-ep1506y-western-blot.jpg "Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (ab68150)")
Key features and details
- Rabbit monoclonal [EP1506Y] to Phospholipase D1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Phospholipase D1 antibody [EP1506Y]
See all Phospholipase D1 primary antibodies -
Description
Rabbit monoclonal [EP1506Y] to Phospholipase D1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
A synthetic peptide corresponding to residues on the N terminus of human Phospholipase D1.
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Positive control
- Hela cell lysate
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1506Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab68150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa). |
Target
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Function
Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic. -
Tissue specificity
Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine. -
Sequence similarities
Belongs to the phospholipase D family.
Contains 1 PH domain.
Contains 2 PLD phosphodiesterase domains.
Contains 1 PX (phox homology) domain. -
Cellular localization
Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 5337 Human
- Entrez Gene: 18805 Mouse
- Omim: 602382 Human
- SwissProt: Q13393 Human
- SwissProt: Q9Z280 Mouse
- Unigene: 382865 Human
- Unigene: 212039 Mouse
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Alternative names
- Choline phosphatase 1 antibody
- hPLD1 antibody
- Phosphatidylcholine-hydrolyzing phospholipase D1 antibody
see all
Images
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Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (ab68150)Anti-Phospholipase D1 antibody [EP1506Y] (ab68150) at 1/500 dilution + Hela cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 120 kDa
Observed band size: 120 kDa
Protocols
Datasheets and documents
References (2)
ab68150 has been referenced in 2 publications.
- Wang Z et al. Binding of PLD2-Generated Phosphatidic Acid to KIF5B Promotes MT1-MMP Surface Trafficking and Lung Metastasis of Mouse Breast Cancer Cells. Dev Cell 43:186-197.e7 (2017). PubMed: 29033361
- Sulzmaier FJ et al. PEA-15 potentiates H-Ras-mediated epithelial cell transformation through phospholipase D. Oncogene 31:3547-60 (2012). PubMed: 22105357
