Overview

  • Product name
    Anti-Phospholipase D1 antibody [EP1506Y]
    See all Phospholipase D1 primary antibodies
  • Description
    Rabbit monoclonal [EP1506Y] to Phospholipase D1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the N terminus of human Phospholipase D1.

  • Positive control
    • Hela cell lysate
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab68150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function
      Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.
    • Tissue specificity
      Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
    • Sequence similarities
      Belongs to the phospholipase D family.
      Contains 1 PH domain.
      Contains 2 PLD phosphodiesterase domains.
      Contains 1 PX (phox homology) domain.
    • Cellular localization
      Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane.
    • Information by UniProt
    • Database links
      • Alternative names
        • Choline phosphatase 1 antibody
        • hPLD1 antibody
        • Phosphatidylcholine-hydrolyzing phospholipase D1 antibody
        • Phospholipase D1 antibody
        • PLD 1 antibody
        • PLD1 antibody
        • PLD1_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (ab68150)
        Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (ab68150)
        Anti-Phospholipase D1 antibody [EP1506Y] (ab68150) at 1/500 dilution + Hela cell lysate at 10 µg

        Secondary
        HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 120 kDa
        Observed band size: 120 kDa

      References

      This product has been referenced in:
      • Sulzmaier FJ  et al. PEA-15 potentiates H-Ras-mediated epithelial cell transformation through phospholipase D. Oncogene 31:3547-60 (2012). Read more (PubMed: 22105357) »

      See 1 Publication for this product

      Publishing research using ab68150? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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