Overview

  • Product name

    Anti-Phospholipase D1 antibody [EP1506Y] - BSA and Azide free
    See all Phospholipase D1 primary antibodies
  • Description

    Rabbit monoclonal [EP1506Y] to Phospholipase D1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Phospholipase D1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa cell lysate.
  • General notes

    ab235997 is the carrier-free version of ab68150 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab235997 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab235997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.
    • Tissue specificity

      Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
    • Sequence similarities

      Belongs to the phospholipase D family.
      Contains 1 PH domain.
      Contains 2 PLD phosphodiesterase domains.
      Contains 1 PX (phox homology) domain.
    • Cellular localization

      Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Choline phosphatase 1 antibody
      • hPLD1 antibody
      • Phosphatidylcholine-hydrolyzing phospholipase D1 antibody
      • Phospholipase D1 antibody
      • PLD 1 antibody
      • PLD1 antibody
      • PLD1_HUMAN antibody
      see all

    Images

    • Anti-Phospholipase D1 antibody [EP1506Y] (ab68150) at 1/500 dilution + HeLa cell lysate at 10 µg

      Secondary
      HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 120 kDa
      Observed band size: 120 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68150).

    References

    ab235997 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab235997.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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