Phospholipid Assay Kit (Colorimetric/Fluorometric) (ab234050)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Sample type: Plasma, Serum
Overview
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Product name
Phospholipid Assay Kit (Colorimetric/Fluorometric)
See all Phospholipid kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Product overview
Phospholipid Assay Kit (Colorimetric/Fluorometric) (ab234050) is a simple, accurate and reproducible way to measure total choline-containing phospholipids in biological samples such as serum and plasma.
This kit provides a high-throughput assay of inhibitors/inducers affecting phospholipid metabolism. The signal is directly proportional to the phospholipid concentration of phospholipids in the sample and can detect phospholipids in the range of 0.1 to 10 nmol per sample.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K351 Phospholipid Assay Kit (Colorimetric/Fluorometric). K351-100 is the same size as the 100 test size of ab234050.
Phospholipids are a major component of lipid bilayer membranes. In addition, phospholipids are biologically important molecules as they are closely involved in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline moiety. In human serum and plasma, almost 95% of the phospholipids are choline-containing phospholipids such as phosphatidylcholine and sphingomyelin. The remaining 5% is mainly choline-free phospholipids (i.e. phosphatidylethanolamine and phosphatidylserine). Phospholipid concentration is considered a disease biomarker as it can be altered in many lung, liver and heart diseases.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer I 1 x 25ml Enzyme Mix IV 1 vial OxiRed Probe 1 x 0.2ml PC Hydrolysis Enzyme 1 vial Phospholipid Standard 1 vial
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab234050 has been referenced in 4 publications.
- Rios Garcia M et al. Trip13 Depletion in Liver Cancer Induces a Lipogenic Response Contributing to Plin2-Dependent Mitotic Cell Death. Adv Sci (Weinh) 9:e2104291 (2022). PubMed: 36031387
- Xiao M et al. Protective effect of Glechoma hederacea extract against gallstone formation in rodent models. BMC Complement Med Ther 21:199 (2021). PubMed: 34261471
- Li R et al. Melatonin Alleviates Low-Temperature Stress via ABI5-Mediated Signals During Seed Germination in Rice (Oryza sativa L.). Front Plant Sci 12:727596 (2021). PubMed: 34646287
- Dybas J et al. Age-related and atherosclerosis-related erythropathy in ApoE/LDLR-/- mice. Biochim Biophys Acta Mol Basis Dis 1866:165972 (2020). PubMed: 32949768