Phospholipid Assay Kit (Colorimetric/Fluorometric) (ab234050)

Overview

  • Product name

    Phospholipid Assay Kit (Colorimetric/Fluorometric)
    See all Phospholipid kits
  • Detection method

    Colorimetric/Fluorometric
  • Sample type

    Serum, Plasma
  • Assay type

    Quantitative
  • Product overview

    Phospholipid Assay Kit (Colorimetric/Fluorometric) (ab234050) is a simple, accurate and reproducible way to measure total choline-containing phospholipids in biological samples such as serum, plasma and exosomes.


    This kit provides a high-throughput assay of inhibitors/inducers affecting phospholipid metabolism. The signal is directly proportional to the phospholipid concentration of phospholipids in the sample and can detect phospholipids in the range of 0.1 to 10 nmol per sample.

  • Notes

    Phospholipids are a major component of lipid bilayer membranes. In addition, phospholipids are biologically important molecules as they are closely involved in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline moiety. In human serum and plasma, almost 95% of the phospholipids are choline-containing phospholipids such as phosphatidylcholine and sphingomyelin. The remaining 5% is mainly choline-free phospholipids (i.e. phosphatidylethanolamine and phosphatidylserine). Phospholipid concentration is considered a disease biomarker as it can be altered in many lung, liver and heart diseases.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Assay Buffer 1 x 25ml
    Development Mix 1 vial
    Hydrolysis Enzyme 1 vial
    Phospholipid Standard 1 vial
    Probe Solution 1 x 0.2ml

Images

  • Normal concentrations in human ranges between 1 and 4 mM.

     

  • Experiments were carried out in triplicate and followed kit protocols.

     

Protocols

References

ab234050 has not yet been referenced specifically in any publications.

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