Recombinant Anti-Phosphomevalonate kinase antibody [EPR15029] (ab199725)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15029] to Phosphomevalonate kinase
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Phosphomevalonate kinase antibody [EPR15029]
See all Phosphomevalonate kinase primary antibodies -
Description
Rabbit monoclonal [EPR15029] to Phosphomevalonate kinase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293 and HeLa whole cell lysates; Human fetal liver, fetal brain, fetal heart and fetal kidney lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15029 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
Target
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Tissue specificity
Heart, liver, skeletal muscle, kidney, and pancreas. Lower level in brain, placenta and lung. -
Pathway
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 10654 Human
- Omim: 607622 Human
- SwissProt: Q15126 Human
- Unigene: 30954 Human
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Alternative names
- hPMK antibody
- HUMPMKI antibody
- OTTHUMP00000035546 antibody
see all
Images
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All lanes : Anti-Phosphomevalonate kinase antibody [EPR15029] (ab199725) at 1/1000 dilution
Lane 1 : HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : Human fetal brain tissue lysate
Lane 4 : Human fetal heart tissue lysate
Lane 5 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Phosphomevalonate kinase antibody [EPR15029] (ab199725) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab199725 has not yet been referenced specifically in any publications.