Key features and details
- Rabbit polyclonal to Phosphoribosyl pyrophosphate amidotransferase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Phosphoribosyl pyrophosphate amidotransferase antibody
See all Phosphoribosyl pyrophosphate amidotransferase primary antibodies
DescriptionRabbit polyclonal to Phosphoribosyl pyrophosphate amidotransferase
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 283-467 of Human Phosphoribosyl pyrophosphate amidotransferase (NP_002694).
- WB: HepG2 and HCT116 whole cell lysates. IF: HeLa whole cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab125864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 57 kDa.|
|ICC/IF||1/100 - 1/1000.|
Tissue specificityUbiquitously expressed.
PathwayPurine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.
Sequence similaritiesIn the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.
Contains 1 glutamine amidotransferase type-2 domain.
- Information by UniProt
- Amidophosphoribosyltransferase [Precursor] antibody
- Amidophosphoribosyltransferase antibody
- ATase antibody
All lanes : Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (ab125864) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
7.5% SDS PAGE
Immunofluorescence analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling Phosphoribosyl pyrophosphate amidotransferase with ab125864 at 1/500 dilution. Costained with Hoechst 33342 (Bottom panel).
ab125864 has not yet been referenced specifically in any publications.