Key features and details
- Rabbit polyclonal to Phosphoserine Aminotransferase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Product nameAnti-Phosphoserine Aminotransferase antibody
See all Phosphoserine Aminotransferase primary antibodies
DescriptionRabbit polyclonal to Phosphoserine Aminotransferase
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human, Pig
Recombinant fragment (His-T7-tag) corresponding to Human Phosphoserine Aminotransferase aa 1-312. (Expressed in E.coli).
MDAPRQVVNFGPGPAKLPHSVLLEIQKELLDYKGVGISVLEMSHRSSDFA KIINNTENLVRELLAVPDNYKVIFLQGGGCGQFSAVPLNLIGLKAGRCAD YVVTGAWSAKAAEEAKKFGTINIVHPKLGSYTKIPDPSTWNLNPDASYVY YCANETVHGVEFDFIPDVKGAVLVCDMSSNFLSKPVDVSKFGVIFAGAQK NVGSAGVTVVIVRDDLLGFALRECPSVLEYKVQAGNSSLYNTPPCFSIYV MGLVLEWIKNNGGAAAMEKLSSIKSQTIYEIIDNSQGFYVSVGGIRASLY NAVTIEDVQKLA
Database link: Q9Y617
- IHC-P: Human cerebrum, glioma and kidney tissues. ICC/IF: HeLa cells. WB: HeLa and HepG2 cell lysates; Pig and mouse brain lysates; Recombinant human Phosphoserine Aminotransferase protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232944 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 40 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
FunctionCatalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.
Tissue specificityExpressed at high levels in the brain, liver, kidney and pancreas, and very weakly expressed in the thymus, prostate, testis and colon.
PathwayAmino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.
Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5.
Involvement in diseaseDefects in PSAT1 are the cause of phosphoserine aminotransferase deficiency (PSATD) [MIM:610992]. PSATD is characterized biochemically by low plasma and cerebrospinal fluid concentrations of serine and glycine and clinically by intractable seizures, acquired microcephaly, hypertonia, and psychomotor retardation.
Sequence similaritiesBelongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.
- Information by UniProt
- EC 18.104.22.168 antibody
- Endometrial progesterone induced protein antibody
- EPIP antibody
Anti-Phosphoserine Aminotransferase antibody (ab232944) at 2 µg/ml + Recombinant human Phosphoserine Aminotransferase protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 40 kDa
Paraffin-embedded human cerebrum tissue stained for Phosphoserine Aminotransferase using ab232944 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human glioma tissue stained for Phosphoserine Aminotransferase using ab232944 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human kidney tissue stained for Phosphoserine Aminotransferase using ab232944 at 10 µg/ml in immunohistochemical analysis. DAB staining.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Phosphoserine Aminotransferase (green) using ab232944 at 20 µg/ml in ICC/IF, followed by FITC staining.
All lanes : Anti-Phosphoserine Aminotransferase antibody (ab232944) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : Pig brain lysate
Lane 4 : Mouse brain lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 40 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232944 has not yet been referenced specifically in any publications.