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Rabbit polyclonal to Phosphoserine Aminotransferase
Predicted to work with:
Mouse, Rat, Rabbit, Cow
Recombinant protein fragment with a sequence corresponding to a region within amino acids 48 and 298 of Human Phosphoserine Aminotransferase (NP_478059).
293T, A431, H1299, HeLa, HepG2, Molt-4 and Raji cell lines. H1299 whole cell lysate for WB and A549 cells for IF.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
1/100 - 1/200.
Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.
Expressed at high levels in the brain, liver, kidney and pancreas, and very weakly expressed in the thymus, prostate, testis and colon.
Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.
Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5.
Involvement in disease
Defects in PSAT1 are the cause of phosphoserine aminotransferase deficiency (PSATD) [MIM:610992]. PSATD is characterized biochemically by low plasma and cerebrospinal fluid concentrations of serine and glycine and clinically by intractable seizures, acquired microcephaly, hypertonia, and psychomotor retardation.
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.
Information by UniProt
EC 220.127.116.11 antibody
Endometrial progesterone induced protein antibody
Western blot - Anti-Phosphoserine Aminotransferase antibody (ab96136)
Anti-Phosphoserine Aminotransferase antibody (ab96136) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 40 kDa 10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-Phosphoserine Aminotransferase antibody (ab96136)
ab96136, at 1/200 staining paraformaldehyde-fixed A549 cells by Immunofluorescence (Panel 1) and merged with DNA probe (Panel 2).
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