Anti-Phosphoserine Aminotransferase antibody (ab96136)
Key features and details
- Rabbit polyclonal to Phosphoserine Aminotransferase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Phosphoserine Aminotransferase antibody
See all Phosphoserine Aminotransferase primary antibodies -
Description
Rabbit polyclonal to Phosphoserine Aminotransferase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow -
Immunogen
Recombinant protein fragment with a sequence corresponding to a region within amino acids 48 and 298 of Human Phosphoserine Aminotransferase (NP_478059).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Molt-4 and Raji cell lines. H1299 whole cell lysate for WB and A549 cells for IF.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. -
Tissue specificity
Expressed at high levels in the brain, liver, kidney and pancreas, and very weakly expressed in the thymus, prostate, testis and colon. -
Pathway
Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.
Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5. -
Involvement in disease
Defects in PSAT1 are the cause of phosphoserine aminotransferase deficiency (PSATD) [MIM:610992]. PSATD is characterized biochemically by low plasma and cerebrospinal fluid concentrations of serine and glycine and clinically by intractable seizures, acquired microcephaly, hypertonia, and psychomotor retardation. -
Sequence similarities
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. - Information by UniProt
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Database links
- Entrez Gene: 29968 Human
- Entrez Gene: 107272 Mouse
- Entrez Gene: 293820 Rat
- Omim: 610936 Human
- SwissProt: Q9Y617 Human
- SwissProt: Q99K85 Mouse
- Unigene: 494261 Human
- Unigene: 592595 Human
see all -
Alternative names
- EC 2.6.1.52 antibody
- Endometrial progesterone induced protein antibody
- EPIP antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab96136 has been referenced in 15 publications.
- Tajan M et al. Serine synthesis pathway inhibition cooperates with dietary serine and glycine limitation for cancer therapy. Nat Commun 12:366 (2021). PubMed: 33446657
- Zhou X et al. Coordinated pyruvate kinase activity is crucial for metabolic adaptation and cell survival during mitochondrial dysfunction. Hum Mol Genet 30:2012-2026 (2021). PubMed: 34169315
- Hennequart M et al. The impact of physiological metabolite levels on serine uptake, synthesis and utilization in cancer cells. Nat Commun 12:6176 (2021). PubMed: 34702840
- Spillier Q et al. Structure-Activity Relationships (SARs) of a-Ketothioamides as Inhibitors of Phosphoglycerate Dehydrogenase (PHGDH). Pharmaceuticals (Basel) 13:N/A (2020). PubMed: 31979167
- Duan W & Liu X PSAT1 Upregulation Contributes to Cell Growth and Cisplatin Resistance in Cervical Cancer Cells via Regulating PI3K/AKT Signaling Pathway. Ann Clin Lab Sci 50:512-518 (2020). PubMed: 32826249