Overview

  • Product name
    Anti-Phosphoserine antibody
    See all Phosphoserine primary antibodies
  • Description
    Rabbit polyclonal to Phosphoserine
  • Host species
    Rabbit
  • Specificity
    Recognize proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA. Antibody slightly cross-reacts with phosphothreonine (about 20%) based on indirect ELISA data.
  • Tested applications
    Suitable for: ELISA, IP, WB, IHC - Wholemount, ICC/IFmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    BSA and KLH-phosphoserine conjugates.

  • Positive control
    • Mouse brain extract for Western Blotting. Synthetic phosphopeptide or phosvitin for ELISA.

Properties

Applications

Our Abpromise guarantee covers the use of ab9332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.
IP Use at 10-0.25 µg/mg of lysate. Use at 10µg/0.25 mg of chromatography fractionated protein sample.
WB Use a concentration of 2 - 4 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.
IHC - Wholemount 1/200.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • Alternative names
    • phospho-Ser antibody
    • pS antibody
    • pSER antibody

Images

  • All lanes : Anti-Phosphoserine antibody (ab9332) at 3 µg/ml (in PBS for 16 hours)

    All lanes : Whole cell lysate of monkey COS7 cells

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : An HRP-conjugated Pig anti-rabbit IgG polyclonal at 1/3000 dilution


    Blocking Step: 10% Milk for 1 hour at room temperature

    See Abreview

  • ab9332 staining Phosphoserine in Human osteosarcoma SAOS-2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized in 0.25% Triton in PBS and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 1% BSA) for 1 hour. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies

    The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.

    The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E). Actin level was measured as an internal standard of cell protein.

    Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies. The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.

References

This product has been referenced in:
  • Papaspyropoulos A  et al. RASSF1A uncouples Wnt from Hippo signalling and promotes YAP mediated differentiation via p73. Nat Commun 9:424 (2018). Read more (PubMed: 29382819) »
  • Kapadia B  et al. Fatty Acid Synthase induced S6Kinase facilitates USP11-eIF4B complex formation for sustained oncogenic translation in DLBCL. Nat Commun 9:829 (2018). Read more (PubMed: 29483509) »
See all 57 Publications for this product

Customer reviews and Q&As

1-10 of 34 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (Estrogen Receptor peptides)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
2 µg
Specification
Estrogen Receptor peptides
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Emiliya Storman

Verified customer

Submitted Dec 07 2017

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (HEPATOCYTES)
Specification
HEPATOCYTES
Treatment
20 ug/ml poly(I:C)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jul 07 2014

Answer

ab9332 is an anti-phosphoserine antibody and its immunogen is a KLH-phosphoserine conjugated to BSA. Since this antibody isn't specific for a particular protein but rather phosphoserine (a single modified amino acid), the species in which this antibody is used is not important. The important thing is that it is guaranteed to work in your application and that the samples used contain proteins which are phosphorylated on serine.

Please note that this phosphoserine antibody is pan-specific, however, steric hindrance by some adjacent amino acids may prevent efficient antibody recognition. We have not performed extensive studies to further characterize the affect of surrounding amino acids.

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab9332.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Rabbit Recombinant protein (CDK8/CycC)
Specification
CDK8/CycC
Type
Indirect

Abcam user community

Verified customer

Submitted Dec 13 2012

Question
Answer

Thank you for contacting us.

Please find attached the requested data.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your enquiry.

I can confirm that these two antibodies (ab9319 and ab9332) recognize modified amino acid residues (phosphotyrosine and phosphoserine respectively) on any proteins including murine samples. You are correct that mouse samples can be used as positive control for both antibodies.

If you need any further assistance, please do not hesitate to contact me.

Read More

Answer

Thank you for your enquiry. I am pleased to hear the customerwould like to make use of our testing discount offer and test ab9332 in IHC-Fr.

DISCOUNT CODE: #####
Expiration date: ######

This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC-Fr and include this code in the Additional Comments section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving the Abreview and wishthe customer luck with their research.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Osteosarcoma SAOS-2)
Specification
Osteosarcoma SAOS-2
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS triton .25%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 03 2012

Answer

Thank you for contacting us.

I am very glad to be of service in helping you to find the correct product for your needs. Ab9332 is guaranteed by our Abpromise to work in WB on mouse tissues.We will be pleased to provide scientific support, replacement or refund should this product not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link:

https://www.abcam.com/index.html?pageconfig=abpromise

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.



Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

1-10 of 34 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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