Overview

  • Product name
    Anti-Phosphoserine antibody (HRP)
    See all Phosphoserine primary antibodies
  • Description
    Rabbit polyclonal to Phosphoserine (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Specificity
    Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Has slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    BSA and KLH-phosphoserine conjugates.

  • Positive control
    • Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.

Properties

Applications

Our Abpromise guarantee covers the use of ab9334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.
ELISA Use a concentration of 1 µg/ml.

Target

  • Relevance
    Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • Alternative names
    • phospho-Ser antibody
    • pS antibody
    • pSER antibody

Images

  • Western blotting of mouse brain extract with ab9334 at a 1/200 dilution on a 12% SDS gel. 50 µg protein loaded in each lane. Antibody incubated for 2 hours incubation at room temperature.

References

This product has been referenced in:
  • Martínez-Florensa M  et al. Serine residues in the LAT adaptor are essential for TCR-dependent signal transduction. J Leukoc Biol 89:63-73 (2011). WB . Read more (PubMed: 20940326) »
  • Zang ZJ  et al. Identification of PP2A as a novel interactor and regulator of TRIP-Br1. Cell Signal 21:34-42 (2009). WB . Read more (PubMed: 18940248) »
See all 9 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer



Es gibt eigentlich kein besonderes Protokoll für diesen Antikörper. Die einzigen Abweichungen von unserem Etablierungsprotokoll sind der Vermerk bezüglich des Blockmittels ("To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.") und die Inkubation für zwei Stunden bei RT. Unser Standardt-Protokoll finden Sie als Anhang an diese Email.

Ich hoffe, dies hilft Ihnen trotzdem weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Answer

Vielen Dank für Ihren Anruf.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456und solltemorgen bei Ihnen ankommen.

Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Answer

Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogens erhalten wir alle nötigen Informationen über Ihre Proben und Ihr Protokoll. Sobald Sie dieses Formular an uns zurückgeschickt haben, werden wir uns Ihr Protokoll ansehen und möglichst Veränderungsvorschläge machen, die Ihre Ergebnisse verbessern werden. Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und er innerhalb der letzten 180 Tage gekauft wurde, werden wir Ihnen gerne einen Ersatz oder eine Gutschrift schicken.




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Answer

Thank you very much for following up with this customer. I am sorry that the results were not improve with the suggestions.

As requested, I have added a new free of charge unit of ab9334 of a different batch to your order xx

I hope the results will be good with the new vial. Please do not hesitate to contact us again should you have any questions or concerns.

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Question
Answer

Thank you for your inquiry.

I am sorry to hear that you are experiencing difficulty with this antibody. Like we said on the phone, please try diluting the primary antibody more to 1:10,000 for 1 -2 hrs at RT, increasing the blocking to 1% BSA with the primary and trying regular ECL instead of the super signal.

If these protocol modifications do not work to improve the signal, please let us know and we will look into replacing or refunding the product in accordance with our Abpromise. We look forward to hearing from you.

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Answer

Thank you for taking time to liaise with the customer and to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

I have reviewed this case and would like to confirm the following points with the customer:

1.) In the image provided, the band of the casein protein seems to have a strange migration. Has the customer tested this casein protein on another gel with the same results?

2.)As general suggestion, I can advise to keep the samples all the time on ice for detection of phosphoprotein. I do also recommend the use of phosphatase inhibitors. As I suppose however that the MS data and the gel have been performed with the same sample of casein, I do acknowledge that the casein protein has been phosphorylated and that therefore the positive control should provide a signal. See also our protocol for phosphoproteins. https://www.abcam.com/ps/pdf/protocols/Western%20blotting%20of%20phospho-proteins%20protocol.pdf

In the event thatthis antibodyhas not worked on different blots detecting the phosphorylated casein, we would be pleased to provide a free of charge replacement orcredit note.

I am looking forward to hear back from you.

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Answer

The phosphoserine antibodies were tested positive for the following phosphopeptides: RREILSRRPpSYR RRALpSLR TPRpSP

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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