Product nameAnti-Phosphoserine antibody (HRP)
See all Phosphoserine primary antibodies
DescriptionRabbit polyclonal to Phosphoserine (HRP)
SpecificitySpecifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Has slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Species independent
BSA and KLH-phosphoserine conjugates.
- Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituent: Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesImmunoaffinity chromatography with phosphoserine-agarose.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab9334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.|
|ELISA||Use a concentration of 1 µg/ml.|
RelevanceChanges in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
- phospho-Ser antibody
- pS antibody
- pSER antibody
This product has been referenced in:
- Martínez-Florensa M et al. Serine residues in the LAT adaptor are essential for TCR-dependent signal transduction. J Leukoc Biol 89:63-73 (2011). WB . Read more (PubMed: 20940326) »
- Zang ZJ et al. Identification of PP2A as a novel interactor and regulator of TRIP-Br1. Cell Signal 21:34-42 (2009). WB . Read more (PubMed: 18940248) »