• Product name
    Anti-Phosphoserine antibody (HRP)
    See all Phosphoserine primary antibodies
  • Description
    Rabbit polyclonal to Phosphoserine (HRP)
  • Host species
  • Conjugation
  • Specificity
    Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Has slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    BSA and KLH-phosphoserine conjugates.

  • Positive control
    • Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.



Our Abpromise guarantee covers the use of ab9334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.
ELISA Use a concentration of 1 µg/ml.


  • Relevance
    Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • Alternative names
    • phospho-Ser antibody
    • pS antibody
    • pSER antibody


  • Western blotting of mouse brain extract with ab9334 at a 1/200 dilution on a 12% SDS gel. 50 µg protein loaded in each lane. Antibody incubated for 2 hours incubation at room temperature.


This product has been referenced in:
  • Martínez-Florensa M  et al. Serine residues in the LAT adaptor are essential for TCR-dependent signal transduction. J Leukoc Biol 89:63-73 (2011). WB . Read more (PubMed: 20940326) »
  • Zang ZJ  et al. Identification of PP2A as a novel interactor and regulator of TRIP-Br1. Cell Signal 21:34-42 (2009). WB . Read more (PubMed: 18940248) »

See all 9 Publications for this product

Customer reviews and Q&As

Es gibt eigentlich kein besonderes Protokoll für diesen Antikörper. Die einzigen Abweichungen von unserem Etablierungsprotokoll sind der Vermerk bezüglich des Blockmittels ("To block use 3%BSA with 0.1% gelatin (do not use milk). We recommen...

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Vielen Dank für Ihren Anruf.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456und ...

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Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogens erhalten wir alle nötigen Informationen über Ihre Proben un...

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Thank you very much for following up with this customer. I am sorry that the results were not improve with the suggestions.

As requested, I have added a new free of charge unit of ab9334 of a different batch to your order xx

I hope t...

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Thank you for your inquiry.

I am sorry to hear that you are experiencing difficulty with this antibody. Like we said on the phone, please try diluting the primary antibody more to 1:10,000 for 1 -2 hrs at RT, increasing the blocking to 1% BS...

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Thank you for taking time to liaise with the customer and to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provi...

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The phosphoserine antibodies were tested positive for the following phosphopeptides: RREILSRRPpSYR RRALpSLR TPRpSP


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