HRP Anti-Phosphoserine antibody (ab9334)
Key features and details
- HRP Rabbit polyclonal to Phosphoserine
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Phosphoserine antibody
See all Phosphoserine primary antibodies -
Description
HRP Rabbit polyclonal to Phosphoserine -
Host species
Rabbit -
Conjugation
HRP -
Specificity
Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Has slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein. -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Phosphoserine conjugated to keyhole limpet haemocyanin. BSA and KLH-phosphoserine conjugates.
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Positive control
- Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.00
Constituent: Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography with phosphoserine-agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.
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ELISA |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use a concentration of 2 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding. |
ELISA
Use a concentration of 1 µg/ml. |
Target
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Relevance
Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family. -
Alternative names
- phospho-Ser antibody
- pS antibody
- pSER antibody
Images
Datasheets and documents
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Datasheet download
References (12)
ab9334 has been referenced in 12 publications.
- Liu X et al. Pre-miRNA Hsa-Let-7a-2: a Novel Intracellular Partner of Angiotensin II Type 2 Receptor Negatively Regulating its Signals. Int J Biol Sci 18:3237-3250 (2022). PubMed: 35637969
- Qu L et al. Plant casein kinases phosphorylate and destabilize a cyclin-dependent kinase inhibitor to promote cell division. Plant Physiol 187:917-930 (2021). PubMed: 34608955
- Wilkes MC et al. Diamond Blackfan anemia is mediated by hyperactive Nemo-like kinase. Nat Commun 11:3344 (2020). PubMed: 32620751
- Martínez-Florensa M et al. Serine residues in the LAT adaptor are essential for TCR-dependent signal transduction. J Leukoc Biol 89:63-73 (2011). WB . PubMed: 20940326
- Zang ZJ et al. Identification of PP2A as a novel interactor and regulator of TRIP-Br1. Cell Signal 21:34-42 (2009). WB . PubMed: 18940248