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I'm seeing a black blot in WB using this antibody.
Asked on Mar 12 2012
Thank you for your inquiry.
I am sorry to hear that you are experiencing difficulty with this antibody. Like we said on the phone, please try diluting the primary antibody more to 1:10,000 for 1 -2 hrs at RT, increasing the blocking to 1% BSA with the primary and trying regular ECL instead of the super signal.
If these protocol modifications do not work to improve the signal, please let us know and we will look into replacing or refunding the product in accordance with our Abpromise. We look forward to hearing from you.
Answered on Mar 12 2012