Product nameAnti-Phosphoserine antibody [PSR-45]
See all Phosphoserine primary antibodies
DescriptionMouse monoclonal [PSR-45] to Phosphoserine
Tested applicationsSuitable for: Dot blot, IP, Indirect ELISA, WBmore details
Species reactivityReacts with: Species independent
- WB: rat brain cortex lysate ( a main 50kDa band is seen by detection with biotin-avidin AEC staining) ELISA: phosphoserine conjugated to BSA
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab9332 as a replacement.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.097% Sodium azide
Concentration information loading...
Purification notesPurified from Ascites
Light chain typeunknown
Our Abpromise guarantee covers the use of ab6639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. Stronger blocking agent percentage is recommended (e.g. 10%), in addition to varying loading amounts and shorter incubation time (1-2h RT)|
RelevanceChanges in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
- phospho-Ser antibody
- pS antibody
- pSER antibody
Anti-Phosphoserine antibody [PSR-45] (ab6639) at 1/200 dilution + Drosophila melanogaster whole tissue lysate 40 ug per lane at 40 µg
Sheep anti mouse (HRP) at 1/2000 dilution
Performed under reducing conditions.
This image is an edited version of an image submitted courtesy of an Abreview submitted on 17 August 2005. We do not have any further information relating to this image.
ab6639 used in Immunoprecipitation.
Whole cell lysate prepared from murine mammary cells was loaded at 250µg.Cells were treated with 5ng/ml TGF beta for varying time periods.
Protein A was used for the Immunoprecipitation step.ab6639 used at a 1/2000 dilution for 10 hours at 4°C.
Western blot was performed with an HRP anti-PCBP1 polyclonal antibody, 1/10000 dilution.
This product has been referenced in:
- Nyati KK et al. TLR4-induced NF-?B and MAPK signaling regulate the IL-6 mRNA stabilizing protein Arid5a. Nucleic Acids Res 45:2687-2703 (2017). WB . Read more (PubMed: 28168301) »
- Liu H et al. Interleukin-4 and interleukin-13 increase NADPH oxidase 1-related proliferation of human colon cancer cells. Oncotarget 8:38113-38135 (2017). Read more (PubMed: 28498822) »