Anti-Phosphoserine antibody [PSR-45] (ab6639)


  • Product name
    Anti-Phosphoserine antibody [PSR-45]
    See all Phosphoserine primary antibodies
  • Description
    Mouse monoclonal [PSR-45] to Phosphoserine
  • Host species
  • Tested applications
    Suitable for: Dot blot, IP, Indirect ELISA, WBmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Other Immunogen Type corresponding to Phosphoserine conjugated to keyhole limpet haemocyanin.


    (Peptide available as ab100812)

  • Positive control
    • WB: rat brain cortex lysate ( a main 50kDa band is seen by detection with biotin-avidin AEC staining) ELISA: phosphoserine conjugated to BSA
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab9332 as a replacement.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.



Our Abpromise guarantee covers the use of ab6639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
IP 1/2000.
Indirect ELISA 1/4000.
WB 1/500 - 1/1000. Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. Stronger blocking agent percentage is recommended (e.g. 10%), in addition to varying loading amounts and shorter incubation time (1-2h RT)


  • Relevance
    Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • Alternative names
    • phospho-Ser antibody
    • pS antibody
    • pSER antibody


  • Anti-Phosphoserine antibody [PSR-45] (ab6639) at 1/200 dilution + Drosophila melanogaster whole tissue lysate 40 ug per lane at 40 µg

    Sheep anti mouse (HRP) at 1/2000 dilution

    Performed under reducing conditions.

    This image is an edited version of an image submitted courtesy of an Abreview submitted on 17 August 2005. We do not have any further information relating to this image.

    See Abreview

  • ab6639 used in Immunoprecipitation.
    Whole cell lysate prepared from murine mammary cells was loaded at 250µg.
    Cells were treated with 5ng/ml TGF beta for varying time periods.
    Protein A was used for the Immunoprecipitation step.
    ab6639 used at a 1/2000 dilution for 10 hours at 4°C.
    Western blot was performed with an HRP anti-PCBP1 polyclonal antibody, 1/10000 dilution.

    See Abreview


This product has been referenced in:
  • Nyati KK  et al. TLR4-induced NF-?B and MAPK signaling regulate the IL-6 mRNA stabilizing protein Arid5a. Nucleic Acids Res 45:2687-2703 (2017). WB . Read more (PubMed: 28168301) »
  • Liu H  et al. Interleukin-4 and interleukin-13 increase NADPH oxidase 1-related proliferation of human colon cancer cells. Oncotarget 8:38113-38135 (2017). Read more (PubMed: 28498822) »

See all 17 Publications for this product

Customer reviews and Q&As

Western blot
Human Cell lysate - whole cell (NSCLC)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
30 µg
0-100 nM volasertib for 2 Hrs
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 11 2017

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your reply.
I am sorry to hear that the suggestions we discussed did not improve the results.

Yes, I'd be happy to replace, credit or refund the antibodies. Please just let me know which option is best for you. In case of a r...

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Thank you for your emails and the additional details you sent.

Yes, the use of PBS is a good point. In case of using anti-phospho antibodies this can actually cause high background due to the presence of phosphate in the buffer. I would sugg...

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Thank you for contacting us.

I am sorry to hear that the 2 antibodies have not been working as expected.

Your protocol looks fine in general. However, both antibodies are directed against ANY phospho-site on a serine or threonine o...

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Thank you for your telephone enquiry last week.

I am sorry to confirm that ab6639 Phosphoserine antibody [PSR-45] has only been tested in rat brain tissue bythe originator, and so we have no images for human samples.

There have, h...

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Thank you for contacting us.

To our knowledge this product has not been tested in IHC. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing disc...

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wis...

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Thank you for contacting Abcam.

Based on the information that you have provided, I do not think that there is any protocol tips that would help resolve the issue, as I think you have tried everything.

Under our Abpromise, which is g...

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Merci pour votre email.

J'ai demandé à notre service comptabilité de mettre en place un avoir référence CN.

Votre référence CN est ******.

Voici les manières dont ...

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