Anti-Phosphothreonine antibody (ab9337)
Key features and details
- Rabbit polyclonal to Phosphothreonine
- Suitable for: WB, IP, ELISA, IHC-Fr
- Reacts with: Mouse, Species independent
- Isotype: IgG
Overview
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Product name
Anti-Phosphothreonine antibody
See all Phosphothreonine primary antibodies -
Description
Rabbit polyclonal to Phosphothreonine -
Host species
Rabbit -
Specificity
Recognize proteins phosphorylated on threonine residues. Not cross-reacted with phosphotyrosine. -
Tested applications
Suitable for: WB, IP, ELISA, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Phosphothreonine conjugated to keyhole limpet haemocyanin.
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Positive control
- Use mouse spleen lysate treated with sodium vanadate or mouse brain lysate for WB. Synthetic phosphopeptide (phosphorylated on threonine) for ELISA.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.00
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use a concentration of 2 µg/ml.
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IP |
Use a concentration of 10 µg/ml. Use at a concentration of 10 µg/mg. Acetone precipitation of the protein extract followed by SDS denaturation is recommended for successful immunoprecipitation.
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ELISA |
Use a concentration of 0.5 µg/ml.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 2 µg/ml. |
IP
Use a concentration of 10 µg/ml. Use at a concentration of 10 µg/mg. Acetone precipitation of the protein extract followed by SDS denaturation is recommended for successful immunoprecipitation. |
ELISA
Use a concentration of 0.5 µg/ml. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Relevance
Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation. Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr. An understanding of transformation by oncogenes and mitogenic processes of growth factors depends on the identification of their substrate and a subsequent determination of how phosphorylation affects their properties. Studies on the role of phosphorylated proteins have been hampered by their low abundance and the problem of distinguishing the various types of phosphorylated proteins. The most common procedure is to label intact cells or small tissue fragments with 32P and subsequently to isolate 32P labeled proteins by conventional biochemical methods. In order to identify the specific amino acids that undergo phosphorylation, additional long and tedious procedures for phosphoamino acid analysis are required. Immunoblotting of cellular proteins with antibodies directed against phosphoamino acids is advantageous as it does not involve 32P labeling, and can therefore be employed to monitor alterations in phosphorylation of specific proteins as they occur in intact organs or the whole animal. Indeed, mono and polyclonal antibodies directed against phosphorylated residues have been generated and found useful as analytical and preparative tools because they enable the rapid identification, quantification and immunoaffinity isolation of phosphorylated cellular proteins.
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (37)
ab9337 has been referenced in 37 publications.
- Zhang B et al. Trehalase is required for sex pheromone biosynthesis in Helicoverpa armigera. Insect Mol Biol 31:334-345 (2022). PubMed: 35084068
- Yang S et al. Spatiotemporal expression of regulatory kinases directs the transition from mitosis to cellular morphogenesis in Drosophila. Nat Commun 13:772 (2022). PubMed: 35140224
- Castillo-Medina RE et al. Light-stimulated dephosphorylation of the BiP-like protein, SmicHSP75 (SBiP1) from Symbiodinium microadriaticum is inhibited by elevated but not low temperature and suggests regulation of the chaperone function. Acta Biochim Pol 69:155-164 (2022). PubMed: 35148474
- Ding C et al. Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection. FASEB J 36:e22468 (2022). PubMed: 35913801
- Liu J et al. Chemokine signaling synchronizes angioblast proliferation and differentiation during pharyngeal arch artery vasculogenesis. Development 149:N/A (2022). PubMed: 36468454