Anti-Phosphothreonine antibody (Biotin) (ab9340)


  • Product name
    Anti-Phosphothreonine antibody (Biotin)
    See all Phosphothreonine primary antibodies
  • Description
    Rabbit polyclonal to Phosphothreonine (Biotin)
  • Host species
  • Conjugation
  • Specificity
    Reacts with free phosphothreonine but does not react with phosphoserine, threonine or phosphotyrosine.
  • Tested applications
    Suitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule conjugated to KLH.

  • Positive control
    • Use mouse brain extract for immunoblotting. Use synthetic phosphopeptide (on threonine) for ELISA.



Our Abpromise guarantee covers the use of ab9340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA(kinase assay): Use at 0.5 µg/mL
    Western blot: Use at 4µg/mL
    IP: Use at 10 µg/250 µg protein sample
    Will detect 100 ng of phosvitin in Western Blots and 0.5 ng of phosvitin with ELISA.
    Can be used for non-radioactive protein kinase assay (ELISA) using biotinylated peptide substrate and immunoblotting of abundant phosphoproteins.
    It is not recommended for immunoblotting of trace cellular phosphoproteins. Acetone precipitation of the protein extract followed by SDS denaturation is recommended for successful immunoprecipitation.
  • Target

    • Relevance
      Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation. Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr. An understanding of transformation by oncogenes and mitogenic processes of growth factors depends on the identification of their substrate and a subsequent determination of how phosphorylation affects their properties. Studies on the role of phosphorylated proteins have been hampered by their low abundance and the problem of distinguishing the various types of phosphorylated proteins. The most common procedure is to label intact cells or small tissue fragments with 32P and subsequently to isolate 32P labeled proteins by conventional biochemical methods. In order to identify the specific amino acids that undergo phosphorylation, additional long and tedious procedures for phosphoamino acid analysis are required. Immunoblotting of cellular proteins with antibodies directed against phosphoamino acids is advantageous as it does not involve 32P labeling, and can therefore be employed to monitor alterations in phosphorylation of specific proteins as they occur in intact organs or the whole animal. Indeed, mono and polyclonal antibodies directed against phosphorylated residues have been generated and found useful as analytical and preparative tools because they enable the rapid identification, quantification and immunoaffinity isolation of phosphorylated cellular proteins.


    This product has been referenced in:
    • Zhang C  et al. Nuclear accumulation of symplekin promotes cellular proliferation and dedifferentiation in an ERK1/2-dependent manner. Sci Rep 7:3769 (2017). WB ; Human . Read more (PubMed: 28630428) »

    See 1 Publication for this product

    Customer reviews and Q&As

    The immunogen is phosphothreonine-KLH conjugates. The antibody is affinity-purified with phosphothrenonine and adsorbed by threonine on Agarose. The antibody recognized beter when the p-thre is next to glycine. Also, at the antiboy concentration...

    Read More

    Thank you very much for clarifying your protocol, I am sorry for the delay and would suggest to try to change the blocking step: try 5% milk 1hr or no blocking , it is possible that the antibody is binding non specifically due to the BSA. I would also...

    Read More

    I'm very sorry to hear you are having a problem with ab9340. Thank you for taking the time to fill in the questionnaire, could you please clarify: -which antibody was used for the immunoprecipitation step(we have on anti-Ets-1 antibody but it has n...

    Read More


    Sign up