Key features and details
- Rabbit monoclonal [RM102] to Phosphothreonine
- Suitable for: ELISA, WB, IP, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-Phosphothreonine antibody [RM102]
See all Phosphothreonine primary antibodies
DescriptionRabbit monoclonal [RM102] to Phosphothreonine
Specificityab238305 reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.
Tested applicationsSuitable for: ELISA, WB, IP, ICC/IFmore details
Species reactivityReacts with: Species independent
Synthetic peptide corresponding to Phosphothreonine conjugated to bovine serum albumin. Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide.
- ICC/IF: Serum-starved A431 cells treated with Calyculin A/Okadaic Acid. WB: Serum-starved A431 cells treated with Calyculin A/Okadaic Acid. IP: A431 cells treated with Calyculin A/Okadaic Acid.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Purification notesPurified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab238305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000.|
|IP||1/100 - 1/500.|
|ICC/IF||1/100 - 1/500.|
RelevancePhosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation. Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr. An understanding of transformation by oncogenes and mitogenic processes of growth factors depends on the identification of their substrate and a subsequent determination of how phosphorylation affects their properties. Studies on the role of phosphorylated proteins have been hampered by their low abundance and the problem of distinguishing the various types of phosphorylated proteins. The most common procedure is to label intact cells or small tissue fragments with 32P and subsequently to isolate 32P labeled proteins by conventional biochemical methods. In order to identify the specific amino acids that undergo phosphorylation, additional long and tedious procedures for phosphoamino acid analysis are required. Immunoblotting of cellular proteins with antibodies directed against phosphoamino acids is advantageous as it does not involve 32P labeling, and can therefore be employed to monitor alterations in phosphorylation of specific proteins as they occur in intact organs or the whole animal. Indeed, mono and polyclonal antibodies directed against phosphorylated residues have been generated and found useful as analytical and preparative tools because they enable the rapid identification, quantification and immunoaffinity isolation of phosphorylated cellular proteins.
Serum-starved A431 (human epidermoid carcinoma cell line) cells treated with Calyculin A/Okadaic Acid stained for Phosphothreonine using ab238305 at a 1/500 dilution in ICC/IF, followed by a PE conjugated secondary antibody (red). Counterstained with DAPI (blue).
Left panel: Non-treated cells.
Right panel: Cells treated with Calyculin A/Okadaic Acid.
All lanes : Anti-Phosphothreonine antibody [RM102] (ab238305) at 1/2000 dilution
Lane 1 : Serum starved A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : Serum starved A431 cells treated with Calyculin A/Okadaic Acid lysate
Immunoprecipitation of Calyculin A/Okadaic Acid treated A431 (Human epidermoid carcinoma cell line) cells by ab238305 at a 1/500 dilution, and then blotted with ab238305.
Lane 1: Whole lysate control.
Lane 2: IP with rabbit IgG control.
Lane 3: IP with ab238305.
ab238305 recognizes Phosphorylated threonine in peptides with different sequences.
It has minimal cross-reactivity with phosphorylated serine.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238305 has not yet been referenced specifically in any publications.