Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (ab9344)
Key features and details
- Rabbit polyclonal to Phosphothreonine-Proline / Phosphoserine-Proline
- Suitable for: ELISA, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody -
Description
Rabbit polyclonal to Phosphothreonine-Proline / Phosphoserine-Proline -
Host species
Rabbit -
Specificity
This antibody specifically reacts to proteins containing phosphothreonine-proline motifs, it also reacts to the phosphoserine-proline motif to a similar degree (pT-P and pS-P motif). The antibody does not react to phosphothreonine, phosphoserine or phosphotyrosine. The antibody will selectively recognize the phosphothreonine-proline motif of MBP, which is the site of phosphorylation by ERK1/ERK2. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Phosphothreonine-Proline/ Phosphoserine-Proline conjugated to keyhole limpet haemocyanin.
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Positive control
- WB: Phosphorylated MBP
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General notes
The antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPKs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography with phosphothreonine-proline on agarose, then immuno-absorption with threonine-proline-NH2 on agarose. -
Primary antibody notes
The antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPKs. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use a concentration of 0.5 µg/ml.
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WB |
Use a concentration of 2 µg/ml.
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Notes |
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ELISA
Use a concentration of 0.5 µg/ml. |
WB
Use a concentration of 2 µg/ml. |
Images
Datasheets and documents
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Datasheet download
References (11)
ab9344 has been referenced in 11 publications.
- Zhang P et al. K63-linked ubiquitination of DYRK1A by TRAF2 alleviates Sprouty 2-mediated degradation of EGFR. Cell Death Dis 12:608 (2021). PubMed: 34117217
- Ryu KJ et al. p38 Stabilizes Snail by Suppressing DYRK2-Mediated Phosphorylation That Is Required for GSK3ß-ßTrCP-Induced Snail Degradation. Cancer Res 79:4135-4148 (2019). PubMed: 31209060
- Murthy T et al. Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin. J Biol Chem 293:10220-10234 (2018). PubMed: 29764937
- Lei T et al. Cyclin K regulates prereplicative complex assembly to promote mammalian cell proliferation. Nat Commun 9:1876 (2018). PubMed: 29760377
- Wang H et al. The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival. Nature 546:426-430 (2017). PubMed: 28607489