Overview

  • Product name

    Anti-Phosphothreonine-Proline antibody
  • Description

    Rabbit polyclonal to Phosphothreonine-Proline
  • Host species

    Rabbit
  • Specificity

    This antibody specifically reacts to proteins containing phosphothreonine-proline motifs, it also reacts to the phosphoserine-proline motif to a similar degree (pT-P and pS-P motif). The antibody does not react to phosphothreonine, phosphoserine or phosphotyrosine. The antibody will selectively recognize the phosphothreonine-proline motif of MBP, which is the site of phosphorylation by ERK1/ERK2.
  • Tested applications

    Suitable for: ELISA, WB, IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule: TpP conjugated to KLH.

  • Positive control

    • WB: Mouse brain lysate ELISA: Phosvitin or casein
  • General notes


    The antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPKs.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Immunoaffinity chromatography with phosphothreonine-proline on agarose, then immuno-absorption with threonine-proline-NH2 on agarose.
  • Primary antibody notes

    The antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPKs.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.
WB Use a concentration of 2 µg/ml.
IP Use at an assay dependent concentration. PubMed: 23269672

References

This product has been referenced in:

See all 8 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

xxxxx

I am very pleased to hear you would like to accept our offer and test ab9344 in IF. The code above will give you £227 off your next order before the expiration date. To redeem this offer, please submit an Abreview for IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site:

https://www.abcam.com/Abreviews

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests whatever they may be. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here:

https://www.abcam.com/abtrial

Read More

Answer

Thank you for your reply.

Kate is currently not in the office but I will try to help with your enquiry as best I can. If you were to test this antibody in IF you would be eligible to take part in the Abtrial testing program.

The way that this program works it that you would purchase ab9344 as normal, test the antibody in IF and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of ab9344 off any product in our catalogue. More information on this offer can be found here:

https://www.abcam.com/abtrial

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab9344.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Answer

Thank you for your message which has been forwarded to the scientific support team.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. I can confirm that ab9344 Phosphothreonine-Proline / Phosphoserine-Proline antibody has been tested in WB and ELISA.

If the product is to be used in an untested application, you may indeed be eligible for our testing discount program if the antibody has not yet been purchased. Please contact me again prior to the purchase by replying to this email and I will be pleased to provide more information.

Alternatively, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me again with any other questions.

Read More

Answer

Thank you for contacting Abcam. The antibody ab9344 is guaranteed to work in ELISAs and so would most probably work in the application that you intend to use it for, however you would have to work out the exact condition for you experiment, but I think it should be ok. If there is anything else I can help you with, please let me kn

Read More

Answer

Thank you for contacting us. We have anti glycine or anti- proline antibodies in our catalogue. The catalogue numbers are ab9344, ab9345, ab37067, ab9442 and ab37087. We unfortunately do not have antibodies available specifically raised against proline-glycine-proline short peptide. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are: www.biocompare.com http://www.biocompare.com/ProductCategories/2045/Antibodies.html Elastin proteins also have repeating units of proline and glycine residues. So probably monoclonal antibodies against part of Elastin will be helpful e.g. ab97963 and ab51599. Although these do not exactly match however it will help to widen your search. I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

Read More

Answer

It is not tested. If there is no steric hindrance, it should be able to do so.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up