Overview

  • Product name
    Phosphotungstic Acid Hematoxylin (PTAH) Stain Kit (Connective Tissue Stain)
  • Sample type
    Tissue sections
  • Product overview

    The PTAH Stain Kit (Connective Tissue Stain) is intended for use in the histological visualization of collagen, striated muscle, glial fibers and collagen without using Zenker’s Fixative containing mercuric chloride.


    This kit may be used on formalin-fixed, paraffin-embedded or frozen sections.


    Other products for staining tissue sections


    Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Notes

    Staining Interpretation

    Fibrin, striated muscle, glial fibers   Blue
    Collagen Brown/Red
    Nuclei Blue

    Control Tissue: Striated muscle.

Properties

Images

  • ab150683 Phosphotungstic Acid Hematoxylin (PTAH) Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human skeletal muscle.

  • ab150683 (Phosphotungstic Acid Hematoxylin (PTAH) Stain) staining striated muscle (blue) and collagen (red) in formalin fixed paraffin embedded normal human muscle

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Staining using ab150683 - Phosphotungstic acid Hematoxylin (PTAH) Stain Kit.

Protocols

References

ab150683 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer



We can recommend to use charged slides for frozen section as thse should prevent the section from coming off the slide.

It is also possible to adapt the protocol to the use of a waterbatch instead of a microwave.


Heated Water Bath

1. Deparaffinize sections if necessary and hydrate to distilled water.

2. Pour Zinc Chloride Solution (10%) into plastic staining jar and set in 60° C. water bath for 10 minutes to equilibrate temperature.

3. Place slide in warmed Zinc Chloride Solution (10%) and incubate for 20 minutes at 60°C.

4. During step 3, pour Ferric Ammonium Sulfate Aqueous Solution into a second plastic staining jar and set in 60° C. water bath for 10 minutes to equilibrate temperature.

5. Rinse slide in running tap water for 1 minute.

6. Rinse in distilled water for 1 minute.

7. Place slide in warmed Ferric Ammonium Sulfate Aqueous Solution and incubate for 5 minutes at 60°C.

8. During step 7, pour Phosphotungstic Acid Hematoxylin Solution into a third plastic staining jar and set in 60°C. water bath for 10 minutes to equilibrate temperature.

9. Rinse slide in running tap water for 2 minutes.

10. Rinse in distilled water for 1 minute.

11. Place slide in warmed Phosphotungstic Acid Hematoxylin Solution and incubate for 60 minutes at 60°C.

12. Differentiate section in 95% Reagent Alcohol. Check section using microscope for proper differentiation.
Note: Graded alcohols will remove some stain.

13. Dehydrate in 3 changes of Absolute Alcohol.

14. Clear in 3 changes of fresh Xylene or Xylene Substitute, and mount in synthetic resin.

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