Anti-Phosphotyrosine antibody [3–365–10] (ab17285)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Phosphotyrosine antibody [3–365–10]
See all Phosphotyrosine primary antibodies -
DescriptionMouse monoclonal [3–365–10] to Phosphotyrosine
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Host speciesMouse
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SpecificityBinds specifically and with a high affinity to proteins with phosphorylated tyrosine residues. Binding can be blocked by phosphotyrosine and phenyl phosphate (IC50:10e-6M, ELISA). Does not crossreact with tyrosine, phosphoserine, phosphothreonine, sulphotyrosine (sulfated (23-25) cholecystokinine) or Na-benzoate The clone number has been updated from (2Q267) to (3–365–10 ) both clone numbers name the same antibody clone.
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Tested applicationsSuitable for: ELISA, WB, IP, ICCmore details
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Species reactivityReacts with: Species independent
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Immunogen
Chemical/ Small Molecule (Hapten O-phopshotyramine)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 0.0536% PBS, 1.45% Sodium chloride -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone number3–365–10
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab17285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent dilution. | |
| WB | Use a concentration of 0.4 - 1 µg/ml. | |
| IP | Use a concentration of 100 µg/ml. | |
| ICC | Use a concentration of 5 - 10 µg/ml. |
Target
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RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
Protocols
References
ab17285 has not yet been referenced specifically in any publications.
