Key features and details
- Rabbit polyclonal to Phosphotyrosine
- Suitable for: ELISA, WB, IP
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-Phosphotyrosine antibody
See all Phosphotyrosine primary antibodies
DescriptionRabbit polyclonal to Phosphotyrosine
SpecificityMinimal to no crossreactivity with phosphothreonine or phosphoserine containing proteins.
Tested applicationsSuitable for: ELISA, WB, IPmore details
Species reactivityReacts with: Species independent
Affinity purified native tyrosine phosphorylated proteins (Human).
- A431 Cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab17302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/5000 - 1/10000.|
|WB||1/1000 - 1/2000.|
|IP||Use at an assay dependent dilution. Use 2.5 µg pwe IP reaction.|
RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
All lanes : Anti-Phosphotyrosine antibody (ab17302)
Lane 1 : Untreated parotid acinar cells
Lane 2 : Parotid acinar cells with 50µM etoposide for 2 hours
Lane 3 : Parotid acinar cells with 5mM H2O2 for 30 min
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated Donkey anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Exposure time: 1 minute
ab17302 has been referenced in 2 publications.
- Wang L et al. RALF1-FERONIA complex affects splicing dynamics to modulate stress responses and growth in plants. Sci Adv 6:eaaz1622 (2020). PubMed: 32671204
- Zhou YB et al. The Receptor-Like Cytoplasmic Kinase STRK1 Phosphorylates and Activates CatC, Thereby Regulating H2O2 Homeostasis and Improving Salt Tolerance in Rice. Plant Cell 30:1100-1118 (2018). PubMed: 29581216