Product nameAnti-Phosphotyrosine antibody
See all Phosphotyrosine primary antibodies
DescriptionRabbit polyclonal to Phosphotyrosine
SpecificityMinimal to no crossreactivity with phosphothreonine or phosphoserine containing proteins.
Tested applicationsSuitable for: ELISA, WB, IPmore details
Species reactivityReacts with: Species independent
Affinity purified native tyrosine phosphorylated proteins (Human).
- A431 Cell lysates.
Storage instructionsShipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab17302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/5000 - 1/10000.|
|WB||1/1000 - 1/2000.|
|IP||Use at an assay dependent dilution. Use 2.5 µg pwe IP reaction.|
RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
All lanes : Anti-Phosphotyrosine antibody (ab17302)
Lane 1 : Untreated parotid acinar cells
Lane 2 : Parotid acinar cells with 50µM etoposide for 2 hours
Lane 3 : Parotid acinar cells with 5mM H2O2 for 30 min
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated Donkey anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Exposure time: 1 minute
ab17302 has not yet been referenced specifically in any publications.