Product nameAnti-Phosphotyrosine antibody
See all Phosphotyrosine primary antibodies
DescriptionRabbit polyclonal to Phosphotyrosine
SpecificityRecognize proteins and peptides phosphorylated on tyrosine residues. No cross-reaction with phosphoserine and threonine.
Tested applicationsSuitable for: ELISA, IP, WB, IHC-P, IHC-Frmore details
Species reactivityReacts with: Species independent
Other Immunogen Type corresponding to Phosphotyrosine conjugated to Keyhole Limpet Haemocyanin (KLH). Iodoacetylphosphotyramine KLH conjugates
- Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA. Human breast cancer tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituent: 0.114% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified with phospho-tyrosine on agarose and competitively eluted with N-acetylated phospho-tyrosine.
Our Abpromise guarantee covers the use of ab9319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. 10 µg/500 µg protein sample.|
RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
All lanes : Anti-Phosphotyrosine antibody (ab9319) at 0.5 µg/ml
Lane 1 : 5 ul of crude protein from of HeLa cell lysate treated with fetal calf serum
Lane 2 : 15 ul of crude protein from HeLa cell lysate treated with fetal calf serum
Lane 3 : 5 ul of crude protein from HeLa cell lysate
Lane 4 : 15 ul of crude protein from HeLa cell lysate
Lysates/proteins at 2 mg/ml per lane.
Immunohistochemistry analysis of human breast cancer with ab9319
This product has been referenced in:
- Ramani K et al. Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation. Am J Pathol 188:640-655 (2018). Read more (PubMed: 29305319) »
- Duan ZZ et al. Protease activated receptor 1 (PAR1) enhances Src-mediated tyrosine phosphorylation of NMDA receptor in intracerebral hemorrhage (ICH). Sci Rep 6:29246 (2016). Read more (PubMed: 27385592) »