Overview

  • Product name

    Anti-Phosphotyrosine antibody (HRP)
    See all Phosphotyrosine primary antibodies
  • Description

    Rabbit polyclonal to Phosphotyrosine (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, IP, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule conjugated to KLH.

  • Positive control

    • Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA.

Properties

Applications

Our Abpromise guarantee covers the use of ab9329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Relevance

    The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Images

  • Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)

    Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)

References

This product has been referenced in:

  • McLaughlin NJ  et al. Platelet-Activating Factor-Mediated Endosome Formation Causes Membrane Translocation of p67phox and p40phox That Requires Recruitment and Activation of p38 MAPK, Rab5a, and Phosphatidylinositol 3-Kinase in Human Neutrophils. J Immunol 180:8192-203 (2008). WB . Read more (PubMed: 18523285) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

We do not have affinity data for this antibody. If you need an HRP-conjugated antibody, I recommend the mouse monoclonal ab16389:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=16389).

More data and references for this clone are available on the datasheet for the unconjugated version, ab10321:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=10321).

Please contact us if you have other questions.

Read More
Application
Western blot
Sample
Mouse Cell lysate - whole cell (CD4+ murine splenoyctes)
Loading amount
50000 cells
Specification
CD4+ murine splenoyctes
Treatment
anti-CD3 and anti-CD28-coated beads
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1

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Verified customer

Submitted Nov 27 2006

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