Key features and details
- Mouse monoclonal [IG2] to Phosphotyrosine
- Suitable for: ELISA, ICC/IF, WB, ICC, Flow Cyt, IP
- Reacts with: Species independent
- Isotype: IgG1
Product nameAnti-Phosphotyrosine antibody [IG2]
See all Phosphotyrosine primary antibodies
DescriptionMouse monoclonal [IG2] to Phosphotyrosine
Specificityab5592 detects phosphotyrosine from most species and tissues. ab5592 will not react with any other phosphorylated amino acids, proteins or nucleotides.
Tested applicationsSuitable for: ELISA, ICC/IF, WB, ICC, Flow Cyt, IPmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to Phosphotyrosine. O-phospho-L-tyrosine and O-phospho-DL-tyramine.
- HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab5592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||1/50 - 1/200.|
|WB||1/100 - 1/1000.|
|ICC||1/50 - 1/200.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use a concentration of 2 µg/ml.|
RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
ab5592 has not yet been referenced specifically in any publications.