The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
Western blot - Anti-Phosphotyrosine antibody [PY20] (HRP) (ab16389)
Anti-Phosphotyrosine antibody [PY20] (HRP) (ab16389) at 1/5000 dilution + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg