Recombinant Anti-Phosphotyrosine antibody [RM111] (ab190824)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM111] to Phosphotyrosine
- Suitable for: WB, IP, ELISA, IHC-P, Flow Cyt, ChIP, ICC/IF
- Reacts with: Species independent
Overview
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Product name
Anti-Phosphotyrosine antibody [RM111]
See all Phosphotyrosine primary antibodies -
Description
Rabbit monoclonal [RM111] to Phosphotyrosine -
Host species
Rabbit -
Specificity
This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with non phosphorylated tyrosine, phospho-serine, and phospho-threonine -
Tested applications
Suitable for: WB, IP, ELISA, IHC-P, Flow Cyt, ChIP, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to Bovine Serum Albumin (BSA).
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Positive control
- Serum starved, EGF treated A431 cells; serum starved, EGF treated A431 cell lysate; EGF treated, A431 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM111 -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000.
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IP |
1/200 - 1/1000.
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
1/200 - 1/500.
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Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ChIP |
1/200 - 1/1000.
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ICC/IF |
1/200 - 1/500.
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Notes |
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WB
1/1000 - 1/5000. |
IP
1/200 - 1/1000. |
ELISA
Use at an assay dependent concentration. |
IHC-P
1/200 - 1/500. |
Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ChIP
1/200 - 1/1000. |
ICC/IF
1/200 - 1/500. |
Target
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Relevance
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
Images
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All lanes : Anti-Phosphotyrosine antibody [RM111] (ab190824) at 1/5000 dilution
Lanes 1 & 3 & 5 : Non treated serum-starved A431 cell lysate
Lanes 2 & 4 & 6 : EGF treated serum-starved A431 cell lysateThe blot was was subjected twice to increasing exposure time in Lanes 3 and 4, and Lanes 5 and 6.
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Immunoprecipitation of EGF-treated A431 cells pulling down Phosphotyrosine-EGFR by ab190824 at 1/1000 dilution; this was followed by western blot with an anti-EGFR rabbit monoclonal antibody.
(1) Whole lysate control;(2) IP by rabbit IgG control;
(3) IP by ab190824
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Immunocytochemistry of serum-starved A431 cells nontreated (Left) or treated with EGF (Right) labeling Phosphotyrosine-EGFR using ab190824 at 1/500 dilution (followed by a PE conjugated secondary antibody, red) and DAPI (blue).
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Analysis of ab190824 reaction to phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. ab190824 only reacted to phosphorylated Tyrosine.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab190824 has been referenced in 1 publication.
- Xu X et al. 17ß-Estradiol nongenomically induces vascular endothelial H2S release by promoting phosphorylation of cystathionine ?-lyase. J Biol Chem 294:15577-15592 (2019). PubMed: 31439665