Overview

  • Product name

    Anti-Phosphotyrosine antibody [RM111]
    See all Phosphotyrosine primary antibodies
  • Description

    Rabbit monoclonal [RM111] to Phosphotyrosine
  • Host species

    Rabbit
  • Specificity

    This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with non phosphorylated tyrosine, phospho-serine, and phospho-threonine
  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-P, Flow Cyt, ChIP, ICC/IFmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to Bovine Serum Albumin (BSA).

  • Positive control

    • Serum starved, EGF treated A431 cells; serum starved, EGF treated A431 cell lysate; EGF treated, A431 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab190824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000.
IP 1/200 - 1/1000.
ELISA Use at an assay dependent concentration.
IHC-P 1/200 - 1/500.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ChIP 1/200 - 1/1000.
ICC/IF 1/200 - 1/500.

Target

  • Relevance

    The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Images

  • All lanes : Anti-Phosphotyrosine antibody [RM111] (ab190824) at 1/5000 dilution

    Lanes 1 & 3 & 5 : Non treated serum-starved A431 cell lysate
    Lanes 2 & 4 & 6 : EGF treated serum-starved A431 cell lysate


    The blot was was subjected twice to increasing exposure time in Lanes 3 and 4, and Lanes 5 and 6.

  • Immunoprecipitation of EGF-treated A431 cells pulling down Phosphotyrosine-EGFR by ab190824 at 1/1000 dilution; this was followed by western blot with an anti-EGFR rabbit monoclonal antibody.
    (1) Whole lysate control;

    (2) IP by rabbit IgG control;

    (3) IP by ab190824

  • Immunocytochemistry of serum-starved A431 cells nontreated (Left) or treated with EGF (Right) labeling Phosphotyrosine-EGFR using ab190824 at 1/500 dilution  (followed by a PE conjugated secondary antibody, red) and DAPI (blue).

  • Analysis of ab190824 reaction to phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. ab190824 only reacted to phosphorylated Tyrosine.

References

ab190824 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190824.
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