Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM111] to Phosphotyrosine
- Suitable for: WB, IP, ELISA, IHC-P, Flow Cyt, ChIP, ICC/IF
- Reacts with: Species independent
Product nameAnti-Phosphotyrosine antibody [RM111]
See all Phosphotyrosine primary antibodies
DescriptionRabbit monoclonal [RM111] to Phosphotyrosine
SpecificityThis antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with non phosphorylated tyrosine, phospho-serine, and phospho-threonine
Tested applicationsSuitable for: WB, IP, ELISA, IHC-P, Flow Cyt, ChIP, ICC/IFmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to Bovine Serum Albumin (BSA).
- Serum starved, EGF treated A431 cells; serum starved, EGF treated A431 cell lysate; EGF treated, A431 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesPurified from an animal origin–free culture supernatant.
ChIP Related Products
Our Abpromise guarantee covers the use of ab190824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|IP||1/200 - 1/1000.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||1/200 - 1/500.|
|Flow Cyt||Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ChIP||1/200 - 1/1000.|
|ICC/IF||1/200 - 1/500.|
RelevanceThe phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
All lanes : Anti-Phosphotyrosine antibody [RM111] (ab190824) at 1/5000 dilution
Lanes 1 & 3 & 5 : Non treated serum-starved A431 cell lysate
Lanes 2 & 4 & 6 : EGF treated serum-starved A431 cell lysate
The blot was was subjected twice to increasing exposure time in Lanes 3 and 4, and Lanes 5 and 6.
Immunoprecipitation of EGF-treated A431 cells pulling down Phosphotyrosine-EGFR by ab190824 at 1/1000 dilution; this was followed by western blot with an anti-EGFR rabbit monoclonal antibody.
(1) Whole lysate control;
(2) IP by rabbit IgG control;
(3) IP by ab190824
Immunocytochemistry of serum-starved A431 cells nontreated (Left) or treated with EGF (Right) labeling Phosphotyrosine-EGFR using ab190824 at 1/500 dilution (followed by a PE conjugated secondary antibody, red) and DAPI (blue).
Analysis of ab190824 reaction to phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. ab190824 only reacted to phosphorylated Tyrosine.
ab190824 has not yet been referenced specifically in any publications.