Recombinant
Recombinant Anti-PHOX2A antibody [EPR9070] (ab134939)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PHOX2A antibody [EPR9070]
See all PHOX2A primary antibodies -
Description
Rabbit monoclonal [EPR9070] to PHOX2A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PHOX2A aa 50-150. The exact sequence is proprietary.
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Positive control
- U87 MG, Neuro 2a, SH SY5Y, HeLa and C6 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9070 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab134939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 30 kDa. | |
| IP | 1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
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Function
May be involved in regulating the specificity of expression of the catecholamine biosynthetic genes. Acts as a transcription activator/factor. Could maintain the noradrenergic phenotype. -
Involvement in disease
Defects in PHOX2A are the cause of congenital fibrosis of extraocular muscles type 2 (CFEOM2) [MIM:602078]. CFEOM encompasses several different inherited strabismus syndromes characterized by congenital restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. CFEOM is characterized clinically by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. CFEOM2 may result from the aberrant development of the oculomotor (nIII), trochlear (nIV) and abducens (nVI) cranial nerve nuclei. -
Sequence similarities
Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 401 Human
- Entrez Gene: 11859 Mouse
- Entrez Gene: 116648 Rat
- Omim: 602753 Human
- SwissProt: O14813 Human
- SwissProt: Q62066 Mouse
- SwissProt: Q62782 Rat
- Unigene: 705937 Human
see all -
Alternative names
- Aristaless homeobox (Drosophila) fibrosis of extraocular muscles congenital 2 autosomal recessive antibody
- Aristaless homeobox gene homolog (Drosophila) antibody
- Aristaless homeobox homolog antibody
see all
Images
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![Western blot - Anti-PHOX2A antibody [EPR9070] (ab134939)](https://www.abcam.com/ps/datasheet/images/134/ab134939/PHOX2A-Primary-antibodies-ab134939-1.jpg)
Western blot - Anti-PHOX2A antibody [EPR9070] (ab134939)All lanes : Anti-PHOX2A antibody [EPR9070] (ab134939) at 1/1000 dilution
Lane 1 : U-87 MG cell lysate
Lane 2 : Neuro-2a cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 30 kDa
Datasheets and documents
References
ab134939 has not yet been referenced specifically in any publications.
