RecombinantRabMAb

Recombinant Anti-PHOX2B antibody [EPR14423] - BSA and Azide free (ab216456)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOX2B antibody [EPR14423] - BSA and Azide free (ab216456)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - BSA and Azide free (ab216456)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - BSA and Azide free (ab216456)
  • Anti-PHOX2B antibody [EPR14423] - BSA and Azide free (ab216456)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14423] to PHOX2B - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PHOX2B antibody [EPR14423] - BSA and Azide free
    See all PHOX2B primary antibodies

  • Description

    Rabbit monoclonal [EPR14423] to PHOX2B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PHOX2B aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q99453

  • Positive control

    • Neuro-2a and SH-SY5Y cell lysates; Human neuroblastoma tissue; HeLa and SH-SY5Y cells.

  • General notes

    Ab216456 is the carrier-free version of ab183741. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab216456 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab216456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.

  • Tissue specificity

    Expressed in neuroblastoma, brain and adrenal gland.

  • Involvement in disease

    Defects in PHOX2B are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. Most mutations consist of 5-10 alanine expansions in the poly-Ala region from amino acids 241-260. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
    Defects in PHOX2B are the cause of susceptibility to neuroblastoma type 2 (NBLST2) [MIM:613013]. A common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system.

  • Sequence similarities

    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • NBLST2 antibody
      • NBPhox antibody
      • Neuroblastoma paired type homeobox protein antibody
      • Neuroblastoma Phox antibody
      • Paired like homeobox 2b antibody
      • Paired mesoderm homeobox 2b antibody
      • Paired mesoderm homeobox protein 2B antibody
      • Paired-like homeobox 2B antibody
      • PHOX 2B antibody
      • PHOX 2B homeodomain protein antibody
      • PHOX2B antibody
      • PHOX2B homeodomain protein antibody
      • PHX2B_HUMAN antibody
      • PMX 2B antibody
      • PMX2B antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOX2B antibody [EPR14423] - BSA and Azide free (ab216456)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOX2B antibody [EPR14423] - BSA and Azide free (ab216456)

      Immunohistochemical analysis of paraffin-embedded Human neuroblastoma tissue labeling PHOX2B  with ab183741 at 1/1000 dilution. The slide is counterstained with Hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183741).

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - BSA and Azide free (ab216456)
      Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - BSA and Azide free (ab216456)

      Immunofluorescence analysis of acetone-fixed HeLa cells labeling PHOX2B  with ab183741 at 1/100 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183741).

    • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - BSA and Azide free (ab216456)
      Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - BSA and Azide free (ab216456)

      Immunofluorescence analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling PHOX2B with ab183741 at 1/100 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183741).

    • Anti-PHOX2B antibody [EPR14423] - BSA and Azide free (ab216456)
      Anti-PHOX2B antibody [EPR14423] - BSA and Azide free (ab216456)

    References (1)

    Publishing research using ab216456? Please let us know so that we can cite the reference in this datasheet.

    ab216456 has been referenced in 1 publication.

    • Ke XX  et al. Phox2B correlates with MYCN and is a prognostic marker for neuroblastoma development. Oncol Lett 9:2507-2514 (2015). IHC ; Mouse . PubMed: 26137098

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