Overview

  • Product name

    Anti-PHOX2B antibody - N-terminal
    See all PHOX2B primary antibodies
  • Description

    Rabbit polyclonal to PHOX2B - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human PHOX2B (N terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
    Database link: Q99453

  • Positive control

    • IP: IMR32 whole cell extract. WB: IMR32, SK-N-AS and SH-SY5Y whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab227719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.
  • Tissue specificity

    Expressed in neuroblastoma, brain and adrenal gland.
  • Involvement in disease

    Defects in PHOX2B are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. Most mutations consist of 5-10 alanine expansions in the poly-Ala region from amino acids 241-260. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
    Defects in PHOX2B are the cause of susceptibility to neuroblastoma type 2 (NBLST2) [MIM:613013]. A common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system.
  • Sequence similarities

    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • NBLST2 antibody
    • NBPhox antibody
    • Neuroblastoma paired type homeobox protein antibody
    • Neuroblastoma Phox antibody
    • Paired like homeobox 2b antibody
    • Paired mesoderm homeobox 2b antibody
    • Paired mesoderm homeobox protein 2B antibody
    • Paired-like homeobox 2B antibody
    • PHOX 2B antibody
    • PHOX 2B homeodomain protein antibody
    • PHOX2B antibody
    • PHOX2B homeodomain protein antibody
    • PHX2B_HUMAN antibody
    • PMX 2B antibody
    • PMX2B antibody
    see all

Images

  • PHOX2B was immunoprecipitated from IMR32 (human neuroblast cell line) whole cell extract with 5 µg ab227719. Western blot was performed from the immunoprecipitate using ab227719. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG IP in IMR32 whole cell extract.

    Lane 2: ab227719 IP in IMR32 whole cell extract.

  • All lanes : Anti-PHOX2B antibody - N-terminal (ab227719) at 1/1000 dilution

    Lane 1 : IMR32 (human neuroblast cell line) whole cell extract
    Lane 2 : SK-N-AS (human neuroblastoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 32 kDa



    12% SDS-PAGE gel.

  • Anti-PHOX2B antibody - N-terminal (ab227719) at 1/1000 dilution + SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell extract at 30 µg

    Predicted band size: 32 kDa



    12% SDS-PAGE gel.

References

ab227719 has not yet been referenced specifically in any publications.

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