Anti-PHOX2B antibody - N-terminal (ab227719)
Key features and details
- Rabbit polyclonal to PHOX2B - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PHOX2B antibody - N-terminal
See all PHOX2B primary antibodies -
Description
Rabbit polyclonal to PHOX2B - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Xenopus laevis -
Immunogen
Synthetic peptide within Human PHOX2B (N terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: Q99453 -
Positive control
- IP: IMR32 whole cell extract. WB: IMR32, SK-N-AS and SH-SY5Y whole cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
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IP |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 32 kDa. |
IP
1/100 - 1/500. |
Target
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Function
Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element. -
Tissue specificity
Expressed in neuroblastoma, brain and adrenal gland. -
Involvement in disease
Defects in PHOX2B are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. Most mutations consist of 5-10 alanine expansions in the poly-Ala region from amino acids 241-260. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
Defects in PHOX2B are the cause of susceptibility to neuroblastoma type 2 (NBLST2) [MIM:613013]. A common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system. -
Sequence similarities
Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8929 Human
- Entrez Gene: 18935 Mouse
- Entrez Gene: 402787 Xenopus laevis
- Omim: 603851 Human
- SwissProt: Q99453 Human
- SwissProt: O35690 Mouse
- Unigene: 87202 Human
- Unigene: 62505 Mouse
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Alternative names
- NBLST2 antibody
- NBPhox antibody
- Neuroblastoma paired type homeobox protein antibody
see all
Images
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PHOX2B was immunoprecipitated from IMR32 (human neuroblast cell line) whole cell extract with 5 µg ab227719. Western blot was performed from the immunoprecipitate using ab227719. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in IMR32 whole cell extract.
Lane 2: ab227719 IP in IMR32 whole cell extract.
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All lanes : Anti-PHOX2B antibody - N-terminal (ab227719) at 1/1000 dilution
Lane 1 : IMR32 (human neuroblast cell line) whole cell extract
Lane 2 : SK-N-AS (human neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 32 kDa12% SDS-PAGE gel.
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Anti-PHOX2B antibody - N-terminal (ab227719) at 1/1000 dilution + SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell extract at 30 µg
Predicted band size: 32 kDa12% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab227719 has been referenced in 1 publication.
- Alleboina S et al. Therapeutically targeting oncogenic CRCs facilitates induced differentiation of NB by RA and the BET bromodomain inhibitor. Mol Ther Oncolytics 23:181-191 (2021). PubMed: 34729395