Key features and details
- Rabbit polyclonal to PHOX2B - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PHOX2B antibody - N-terminal
See all PHOX2B primary antibodies
DescriptionRabbit polyclonal to PHOX2B - N-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Xenopus laevis
Synthetic peptide within Human PHOX2B (N terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: Q99453
- IP: IMR32 whole cell extract. WB: IMR32, SK-N-AS and SH-SY5Y whole cell extracts.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 32 kDa.|
|IP||1/100 - 1/500.|
FunctionInvolved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.
Tissue specificityExpressed in neuroblastoma, brain and adrenal gland.
Involvement in diseaseDefects in PHOX2B are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. Most mutations consist of 5-10 alanine expansions in the poly-Ala region from amino acids 241-260. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
Defects in PHOX2B are the cause of susceptibility to neuroblastoma type 2 (NBLST2) [MIM:613013]. A common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system.
Sequence similaritiesBelongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- NBLST2 antibody
- NBPhox antibody
- Neuroblastoma paired type homeobox protein antibody
PHOX2B was immunoprecipitated from IMR32 (human neuroblast cell line) whole cell extract with 5 µg ab227719. Western blot was performed from the immunoprecipitate using ab227719. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in IMR32 whole cell extract.
Lane 2: ab227719 IP in IMR32 whole cell extract.
All lanes : Anti-PHOX2B antibody - N-terminal (ab227719) at 1/1000 dilution
Lane 1 : IMR32 (human neuroblast cell line) whole cell extract
Lane 2 : SK-N-AS (human neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 32 kDa
12% SDS-PAGE gel.
Anti-PHOX2B antibody - N-terminal (ab227719) at 1/1000 dilution + SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell extract at 30 µg
Predicted band size: 32 kDa
12% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227719 has not yet been referenced specifically in any publications.