Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal  to PHPT1
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-PHPT1 antibody 
See all PHPT1 primary antibodies
DescriptionRabbit monoclonal  to PHPT1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human PHPT1 aa 2-125.
Database link: Q9NRX4-1
- WB: HepG2, THP1 and MCF-7 cell lysate. IP: HepG2 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab276061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 14 kDa.|
|IP||Use a concentration of 1 - 5 µg/ml.|
FunctionExhibits phosphohistidine phosphatase activity.
Tissue specificityExpressed abundantly in heart and skeletal muscle.
Sequence similaritiesBelongs to the janus family.
- Information by UniProt
- 14 kDa phosphohistidine phosphatase antibody
- CGI 202 antibody
- HSPC141 antibody
PHPT1 was immunoprecipitated from 0.5 mg of HeLa whole cell lysate using ab276061 at 1/100 dilution followed by Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Develope dusing the ECL technique.
All lanes : Anti-PHPT1 antibody  (ab276061) at 1/500 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : THP-1 whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution
Predicted band size: 14 kDa
Performed under reducing conditions.
ab276061 has not yet been referenced specifically in any publications.