Key features and details
- Rabbit polyclonal to PHPT1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PHPT1 antibody
See all PHPT1 primary antibodies
DescriptionRabbit polyclonal to PHPT1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
- IHC-P: Human brain and liver tissue. WB: Recombinant human PHPT1 protein; MCF7, A549 and HEK-293T whole cell lysate; mouse kidney tissue lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab233116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 14 kDa.|
FunctionExhibits phosphohistidine phosphatase activity.
Tissue specificityExpressed abundantly in heart and skeletal muscle.
Sequence similaritiesBelongs to the janus family.
- Information by UniProt
- 14 kDa phosphohistidine phosphatase antibody
- CGI 202 antibody
- HSPC141 antibody
All lanes : Anti-PHPT1 antibody (ab233116) at 1 µg/ml
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate.
Lane 2 : A549 (human lung carcinoma cell line) cell lysate.
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate.
Lane 4 : Mouse kidney tissue lysate.
Predicted band size: 14 kDa
Anti-PHPT1 antibody (ab233116) at 2 µg/ml + Recombinant human PHPT1 protein.
Predicted band size: 14 kDa
Formalin-fixed, paraffin-embedded human liver tissue stained for PHPT1 using ab233116 at 20µg/mL in immunohistocehmical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human brain tissue stained for PHPT1 using ab233116 at 20µg/mL in immunohistocehmical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233116 has not yet been referenced specifically in any publications.