Product nameAnti-PHRF1 antibody
See all PHRF1 primary antibodies
DescriptionRabbit polyclonal to PHRF1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide corresponding to a region between residues 1400 and 1450 of Human PHRF1 (NP_065952.1).
- HeLa, 293T and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 179 kDa (predicted molecular weight: 179 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
RelevancePHRF1 contains 1 PHD-type zinc finger and 1 RING-type zinc finger. It may interact with POLR2A (via the C-terminal domain).
- AA673488 antibody
- CTD binding SR like protein rA9 antibody
- KIAA1542 antibody
All lanes : Anti-PHRF1 antibody (ab85974) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 179 kDa
Observed band size: 179 kDa
Exposure time: 10 seconds
Detection of PHRF1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85974 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.