Recombinant Anti-PHYHD1 antibody [EPR13313(B)] (ab184174)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13313(B)] to PHYHD1
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PHYHD1 antibody [EPR13313(B)]
See all PHYHD1 primary antibodies -
Description
Rabbit monoclonal [EPR13313(B)] to PHYHD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver andHuman fetal kidney lysates. HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13313(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
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ICC/IF |
1/250 - 1/500.
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Flow Cyt (Intra) |
1/400.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa. |
ICC/IF
1/250 - 1/500. |
Flow Cyt (Intra)
1/400. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Probably acts as alpha-ketoglutatare-dependent dioxygenase. -
Sequence similarities
Belongs to the PhyH family. PHYHD1 subfamily. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 254295 Human
- SwissProt: Q5SRE7 Human
- Unigene: 709447 Human
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Alternative names
- MGC16638 antibody
- OTTHUMP00000022302 antibody
- OTTHUMP00000022303 antibody
see all
Images
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All lanes : Anti-PHYHD1 antibody [EPR13313(B)] (ab184174) at 1/10000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 32 kDa5% NFDM/TBST blocking/diluting buffer
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Intracellular Flow Cytometry analysis of HepG2 cells fixed in 2% paraformaldehyde labeling PHYHD1 with ab184174 at 1/400 dilution (red). Goat andti rabbit IgG (FITC) was used at 1/150 dilution as secondary antibody. Rabbit monoclonal IgG was used as an isotype control (green).
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Immunofluorescent analysis of HepG2 cells fixed in 4% paraformaldehyde labeling PHYHD1 with ab184174 at 1/500 dilution. Goat anti-rabbit IgG(Alexa Fluor® 555) was used at 1/200 dilution for secondary antibody and the cells were counterstained with DAPI.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab184174 has not yet been referenced specifically in any publications.